Phylogenetic Variation of Chelonid Alphaherpesvirus 5 (ChHV5) in Populations of Green Turtles Chelonia mydas along the Queensland Coast,Australia |
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Authors: | E. Ariel F. Nainu K. Jones K. Juntunen I. Bell J. Gaston |
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Affiliation: | 1. College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland 4811, Australiaellen.ariel@jcu.edu.au;3. College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland 4811, Australia;4. Faculty of Pharmacy, Hasanuddin University, South Sulawesi 90245. Makassar, Indonesia;5. Department of Environment and Heritage Protection, Post Office Box 5597, Townsville, Queensland 4810, Australia;6. Gudjuda Reference Group Aboriginal Corporation, Corner First Street and Georgees Road, Home Hill, Queensland 4806, Australia |
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Abstract: | AbstractSea turtle fibropapillomatosis (FP) is a disease marked by the proliferation of benign but debilitating cutaneous and occasional visceral tumors, likely to be caused by chelonid alphaherpesvirus 5 (ChHV5). This study presents a phylogeny of ChHV5 strains found on the east coast of Queensland, Australia, and a validation for previously unused primers. Two different primer sets (gB-1534 and gB-813) were designed to target a region including part of the UL27 glycoprotein B (gB) gene and part of UL28 of ChHV5. Sequences obtained from FP tumors found on juvenile green turtles Chelonia mydas (<65 cm curved carapace length) had substantial homology with published ChHV5 sequences, while a skin biopsy from a turtle without FP failed to react in the PCRs used in this study. The resulting sequences were used to generate a neighbor-joining tree from which three clusters of ChHV5 from Australian waters were identified: north Australian, north Queensland, and Queensland clusters. The clusters reflect the collection sites on the east coast of Queensland with a definitive north–south trend.Received October 22, 2016; accepted May 7, 2017 Published online July 26, 2017 |
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