白绒山羊性控胚胎生产及移植应用研究初探

应用X性控冷冻精液对超排供体白绒山羊进行人工授精，生产性控胚胎并进行鲜胚移植。结果发现，①用性控冻精和鲜精输精的供体母羊，平均卵子回收数分别为13.3和12.5枚，其中性控冻精组受精卵比例为29.6%（55/186），极显著低于鲜精组94.0%（281/299）的比例（P<0.01）；②性控胚胎和普通胚胎移植受体母羊产羔率分别为42.2% 和58.1% ，二者差异极显著（P<0.01）；③性控胚胎移植受体母羊所产羔羊雌性所占百分数为100%，极显著高于普通胚胎移植（P<0.01）。表明通过白绒山羊X、Y精子分离、人工授精生产性控胚胎，同时结合胚胎移植技术，可以达到按照生产实际中的意愿得到性别控制子代白绒山羊的目的。

Preliminary Studies on the Production of Gender Preselected Embryos and the Embryos Transfer in White Cashmere Goat

The superovulated donors of white cashmere goat were artificially inseminated (AI) with frozen sexed X semen to produce gender preselected embryos, then performed fresh embryo transfer. Results: ①The average numbers of ovum collection from the group of AI with frozen sorted semen and the group of fresh unsorted semen were 13.3 and 12.5 respectively. The rates of fertilization of these two groups were 29.6%(55/186) and 94.0% (281/299), the former was significantly lower than the latter(P<0.01). ②The lambing rates of gender preselected embryos transferring and untreated embryos transferring were 42.2% and 58.1% respectively. There was significant difference between the two groups (P<0.01). ③The rate of correct offspring in gender preselected embryos transfer group was 100%, which was significantly higher than untreated groups’57.4%(P<0.01). The results was proved the feasibility of gender preselection in offspring of white cashmere goat by sorted X and Y sperm, artificial insemination to produce embryos, and embryo transfer to obtain the kidlets.