| 台湾引进甜杨桃的两种真菌病原鉴定 |
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| 引用本文： | 刘韬,肖荣凤,刘波,黄素芳,阮传清,林捷.台湾引进甜杨桃的两种真菌病原鉴定[J].福建农业学报,2011,26(3):409-414. |
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| 作者姓名： | 刘韬 肖荣凤 刘波 黄素芳 阮传清 林捷 |
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| 作者单位： | 1. 福建省农业科学院生物技术研究所,福建福州,350003
2. 福建省农业科学院农业生物资源所,福建福州,350003 |
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| 基金项目： | 福建省科技计划重点项目，公益性行业(农业)科研专项项目 |
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| 摘 要： | 对福建闽南地区种植的从台湾引进的甜杨桃进行真菌性病害的鉴定.在病害症状和病症组织病理学鉴定的基础上,进行菌株的rDNA-ITS区序列PCR扩增,测序结果在GenBank中进行同源性搜索,下载部分具有代表性属或种的ITS序列,利用软件MEGA4构建分子系统发育树；按照柯赫氏法则对病原物进行了致病性测定,回接后的叶片或果实...
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| 关 键 词： | 甜杨桃 病害 鉴定 拟茎点霉 胶孢炭疽菌 |
Identification of Two Fungal Pathogens in Sweet Carambola from Taiwan |
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| LIU tao,XIAO Rong-feng,LIU Bo,HUANG Su-fang,RUAN Chuan-qing,LIN Jie.Identification of Two Fungal Pathogens in Sweet Carambola from Taiwan[J].Fujian Journal of Agricultural Sciences,2011,26(3):409-414. |
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| Authors: | LIU tao XIAO Rong-feng LIU Bo HUANG Su-fang RUAN Chuan-qing LIN Jie |
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| Institution: | 1(1.Institute of Biotechnology,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;2.Agricultural Bio-resources Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China) |
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| Abstract: | Fungal diseases of the sweet carambola,Averrhoa carambola L.,introduced from Taiwan were investigated.The infected samples were collected from southern Fujian.Two fungal pathogens were identified by using histopathology and the ribosomal DNA internal transcribed spacer(ITS) sequencing.By comparing with the corresponding sequences in GenBank,the phylogenetic trees for the strains,SC-01 and SC-06,were constructed.The two isolates were re-inoculated by the Koch’s rule to the leaves and fruits to produce identical symptoms as observed in the field.Subsequently,it was confirmed that the leaf spot was caused by Phomopsis sp.,and the leaf and fruits anthracnose by Collotrichum gloeosporioides. |
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| Keywords: | sweet carambola diseases identify Phomopsis sp Colletotrichum gloeosporioides |
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