一例引起欧洲鳗鲡死亡的嗜水气单胞菌分离与鉴定

从患病欧洲鳗鲡内脏组织中分离获得1株菌FZ01,对该菌进行了形态学、药物敏感性试验、动物感染试验和血清型分析,并进行了VITEK全自动微生物分析系统鉴定.结果显示:该菌为革兰氏阴性短杆菌,端圆,散在,TCBS琼脂上呈细小黄色菌落.VITEK全自动微生物系统鉴定该分离株为致病性嗜水气单胞菌.动物感染试验证明,对欧鳗和小白...

Isolation and Identification of a Pathogen from an Infected European Eel (Anguilla Anguilla)

The strain FZ01 was isolated from a diseased Anguilla Anguilla taken from a fish farm suffering from an outbreak of unknown pathogen.The virulence,morphological,physiological and biochemical characteristics and serotype of the FZ01 were investigated.Oxidase test,catalase test and a glucose fermentation test were done by A VITEK automatic microbiological identification analysis.The results showed that the strain FZ01 was pathogenic for both eel and mouse.The fatality rate of 2×107CFU for eel was 100% in 48 h.It was confirmed as the causative agent of the epidemic.FZ01 was Gram-negative bacilli,and the results showed that it was Aeromonas hydrophila.Tests of the pathogens susceptibility to antibiotics demonstrated its sensitivity to Enrofloxacin,tetracycline,and ciprofloxacin,but resistance to amoxicillin and sulfamethoxazole.Serologic tests showed that this strain′s cell agglutination titer with the Fujian Province serotype O∶Ah10501 was 1∶27.These results suggested that we should take full account of serotype features in disease control process,and select the appropriate drugs and immune agents.