In vitro inhibitory effect of trichostatin A on canine grade 3 mast cell tumor |
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| Authors: | Marcia Kazumi Nagamine Daniel S Sanches Katia C Pinello Luciana Neves Torres Gregory Mennecier Andréia O Latorre Heidge Fukumasu Maria Lucia Zaidan Dagli |
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| Institution: | 1.Laboratory of Experimental Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science,University of S?o Paulo,S?o Paulo,Brazil;2.Laboratory of Pharmacology and Toxicology, Department of Pathology, School of Veterinary Medicine and Animal Science,University of S?o Paulo,S?o Paulo,Brazil;3.Laboratory of Molecular Morfophysiology and Development, Department of Basic Science, Faculty of Animal Science (Zootecnia) and Food Engineering,University of S?o Paulo,Pirassununga,Brazil |
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| Abstract: | Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect skin and soft tissue in dogs. Because mast cell tumors
present a great variety of clinical appearance and behavior, their treatment becomes a challenge. Trichostatin A (TSA), an
antifungal antibiotic, has shown inhibitory effects on the proliferation and induction of apoptosis in various types of cancer
cells. In order to evaluate the potential of trichostatin A as a therapeutic drug, cells of grade 3 MCT were cultured and
treated with concentrations of 1 nM to 400 nM of TSA. MTT assay and trypan blue exclusion assays were performed to estimate
cell growth and cell viability, and cell cycle analysis was evaluated. TSA treatment showed a reduction in numbers of viable
cells and an increase of cell death by apoptosis. The cell cycle analysis showed an increase of hypodiploid cells and a reduction
of G0/G1 and G2/M –phases. According to these results, trichostatin A may be an interesting potential chemotherapeutic agent
for the treatment of canine MCT. |
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