变异型猪繁殖与呼吸综合征病毒FJ06A毒株的分离鉴定和致病性

从某一疑似发生无名“高热病”猪场的病料中分离到一株病毒,经病毒RT-PCR鉴定、生物学特性分析、病毒结构蛋白和非结构蛋白NSP2基因序列分析及对不同日龄猪感染试验的结果表明:分离毒株为变异型猪繁殖与呼吸综合征病毒(PRRSV),该病毒具有高致病性,病毒能在Marc-145细胞上增殖并产生病变,其结构蛋白ORF2-ORF...

Isolation, identification and pathogenicity of variant porcine reproductive and respiratory syndrome viruses FJ06A strain

One strain of porcine reproductive and respiratory syndrome virus(PRRSV) was isolated from the tissue of "high fever" pigs in a farm.It was identified as variant PRRSV by biological characteristics detection,RT-PCR,and sequences analysis of structural protein and NSP2 gene.The PRRSV negative pigs were inoculated with this strains and showed obvious clinical symptom and died.The sequences analysis of ORF2-ORF7 genes showed that the homology was from 87.6% to 97.1% comparing with PRRSV VR2332,and it was from 88.3% to 99.6% comparing with the prevalent strains from other provinces in China,but it was from 59.8% to 79.4% comparing with LV strain in amino acids sequence.Sequences analysis of NSP2 gene indicated that the strain shared a common feature of 31 amino acids deletion.It was lowly homologous to strain VR2332,with homology ranging 78.1%,and highly to the prevalent strains from some provinces of China,with homology ranging from 90.7% to 99.6%.