应用测试片快速检测食品中的大肠杆菌O157：H7

目的应用大肠杆菌O157:H7测试片快速检测食品中的大肠杆菌O157:H7。方法对大肠杆菌O157:H7测试片的各项指标及影响因素进行测试,并将其应用于食品检测。结果大肠杆菌O157:H7测试片的检测灵敏度高,其对纯菌的检测低限可达3cfu/mL;特异性较强,与鼠伤寒沙门氏菌、志贺氏菌等21种非目的菌无交叉反应;快速,24h内可报告阴性检测结果。应用该测试片检测各种食品206份,检测结果与SN标准的符合率达到98.5%。结论应用测试片检测食品中的大肠杆菌O157:H7具有快速、方便、经济、无需昂贵设备等优点。该测试片可适应于食品中大肠杆菌O157:H7的快速初筛。

Quick Detection of Escaerichia coil O157:H7 in Food by Detective Slips

Objective To detect Escherichia coli O157:H7 in food by detective slips rapidly. Method The detectabilities and effect of factors on Escherichia coli O157:H7 detective slip were studied, and the detective slips were applied to detect Escherichia coli O157:H7 in food. Result The results showed that the detective slip was high sensitive, strong specific and rapid, it could detect 3cfu/mL Escherichia coli O157:H7 in pure culture, had no cross-reaction with 21 kind of non-target bateria such as S.typhimurium and Shigella, and could take 24h to get negative result. The detective slips were applied to detect Escherichia coli O157:H7 in 206 food samples, and the rates of accordance between the detective slips and SN were 98.5%. Conclusion The detective slips could be applied to detect Escherichia coli O157:H7 in food primarily with rapid, simple, economic and without the requirements of expensive equipment.