The EST Analysis of A Suppressive Subtraction cDNA Library of Chinese Wild Vitis pseudoreticulata Inoculated with Uncinula necator |
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| Authors: | SHI Jiang-li WANG Yue-jin ZHU Zi-guo ZHANG Chao-hong |
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| Institution: | Key Laboratory of Horticultural Plant Germplasm Utilization in Northwest China, Ministry of Agriculture/Shaanxi Key Laboratory of Molecular Biology for Agriculture/College of Horticulture, Northwest A＆F University, Yangling 712100, P.R.China |
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| Abstract: | Uncinula necator is a worldwide serious fungus disease causing annual heavy lost on grapevine production.In order to get more informations on defense related EST sequences and help breeding program,we constructed and characterized a suppression subtractive hybridization cDNA library with artificially inoculated leaves and control Chinese wild Vitis pseudoreticulata clone Baihe-35-1,which is highly resistant to powdery mildew.In the library,the length of 58 EST fragments known as putative functions varied from 130 to 800 bp,and 60% of the ESTs exhibited high similarity to known sequences in database of GenBank with BLASTX analysis.These genes were involved in stress/defense response,detoxification,signal transduction,disease defense,and etc.,and 14 ESTs remained unknown or hypothetical proteins,which may be new genes.The experiment provided an important basis for studying the disease-resistance mechanism and obtaining the genes for the aim of improving grapevine powdery mildew resistance. |
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| Keywords: | Chinese wild Vitis pseudoreticulata disease resistance SSH cDNA library EST sequence |
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