抱茎苦荬菜药材中总黄酮和腺苷的含量测定

目的：建立测定抱茎苦荬菜药材中总黄酮和腺苷的方法。方法：采用UV—VIS法测定总黄酮的含量。采用RP—HPLC法测定腺苷的含量，色谱条件为：XDB—C18（150mm×4．6mm，5μm）色谱柱，以乙腈-水（体积比为6：94）为流动相，检测波长为260nm，流速为1ml／min^-1。结果：腺苷进样量在0．072—3．6μg范围内线性关系良好（r=0．999），平均回收率为100．3％，相对标准差（RSD）为1．35％。结论：方法准确、可靠，适合测定抱茎苦荬菜药材中总黄酮和腺苷的含量。

quantitative determination of total flavonoids and adenosine in the Ixeris sonchifolia Hance

Objective To develop a method for the determination of the content of adenosine in Ixeris sonchifolia Hance. Methods The content of total flavonoids was determined by using UV - Vis method. The content of adenosine was determined by using HPLC method. It was seperated on a XDB -Cls （150 mm×4.6 mm,5bcm）colurrm with CAN- H20（6：94） as the mobile phase,The UV detective wavelength was set at 260nm,the flow rate was 1ml/min. Results The linear calibration curves are obtai -ned in the ranges of 0.072 -3.61μg. The average recovery is 100.3 and RSD is 1.35%. Conclusions The method is simple and accurate,It can be applied to the determination of content of adenosine in Ixeris sonchifolia Hance.