| 3,5-二硝基水杨酸比色法测定麦冬多糖含量 |
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| 引用本文： | 王红英,钱斯日古楞,赵前程,藏姝,金凤燮.3,5-二硝基水杨酸比色法测定麦冬多糖含量[J].沈阳农业大学学报,2005,36(5):628-630. |
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| 作者姓名： | 王红英 钱斯日古楞 赵前程 藏姝 金凤燮 |
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| 作者单位： | 1. 大连轻工业学院,生物与食品工程学院,辽宁,大连,116034
2. 大连水产学院,食品工程系,辽宁,大连,116023 |
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| 基金项目： | 大连市科技攻关项目（2004B3SF175） |
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| 摘 要： | 采用3,5-二硝基水杨酸(DNS)比色法测定麦冬多糖含量。结果表明,麦冬多糖占麦冬总干重的40.4mg.g-1,RSD=2.48%(n=5),总糖供试液和单糖供试液的平均回收率分别是100.4%(RSD=0.17%)和101.1%(RSD=0.62%)。试验表明,DNS比色法测定多糖含量简单易行,且重现性好,可作为麦冬多糖含量的常规测定方法。
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| 关 键 词： | 麦冬 多糖 3 5-二硝基水杨酸比色法 |
| 文章编号： | 1000-1700(2005)05-0628-03 |
| 收稿时间： | 10 21 2004 12:00AM |
| 修稿时间： | 2004年10月21 |
Determination of Polysaccharide in Ophiopogon japonicus with 3,5-Dinitrosalicylic Acid Colorimeitry |
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| WANG Hong-ying,QIAN Srguleng,ZHAO Qian-cheng,ZANG Sbu,JIN Feng-xie.Determination of Polysaccharide in Ophiopogon japonicus with 3,5-Dinitrosalicylic Acid Colorimeitry[J].Journal of Shenyang Agricultural University,2005,36(5):628-630. |
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| Authors: | WANG Hong-ying QIAN Srguleng ZHAO Qian-cheng ZANG Sbu JIN Feng-xie |
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| Institution: | WANG Hong-ying1,QIAN Srguleng1,ZHAO Qian-cheng2,ZANG Shu1,JIN Feng-xie1 |
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| Abstract: | 3,5-Dinitrosalicylic acid(DNS) colorimetry was used to determine the content of polysaccharide in Ophiopogon japonicus.The result showed that the content of polysaccharide in Ophiopogon japonicus was 40.4 mg.g-1 with RSD=2.48%(n=5);average recoveries of total sugar solution and monosaccharide solution in the test were 100.4% with RSD,0.17%(n=5) and 101.1% with RSD,0.62%(n=5) respectively.This method could be used to determine the contents of polysaccharide in Ophiopogon japonicus. |
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| Keywords: | Ophiopogon japonicus polysaccharide 3 5-dinitrosalicylic acid |
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