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3,5-二硝基水杨酸比色法测定麦冬多糖含量
引用本文:王红英,钱斯日古楞,赵前程,藏姝,金凤燮.3,5-二硝基水杨酸比色法测定麦冬多糖含量[J].沈阳农业大学学报,2005,36(5):628-630.
作者姓名:王红英  钱斯日古楞  赵前程  藏姝  金凤燮
作者单位:1. 大连轻工业学院,生物与食品工程学院,辽宁,大连,116034
2. 大连水产学院,食品工程系,辽宁,大连,116023
基金项目:大连市科技攻关项目(2004B3SF175)
摘    要:采用3,5-二硝基水杨酸(DNS)比色法测定麦冬多糖含量。结果表明,麦冬多糖占麦冬总干重的40.4mg.g-1,RSD=2.48%(n=5),总糖供试液和单糖供试液的平均回收率分别是100.4%(RSD=0.17%)和101.1%(RSD=0.62%)。试验表明,DNS比色法测定多糖含量简单易行,且重现性好,可作为麦冬多糖含量的常规测定方法。

关 键 词:麦冬  多糖  3  5-二硝基水杨酸比色法
文章编号:1000-1700(2005)05-0628-03
收稿时间:10 21 2004 12:00AM
修稿时间:2004年10月21

Determination of Polysaccharide in Ophiopogon japonicus with 3,5-Dinitrosalicylic Acid Colorimeitry
WANG Hong-ying,QIAN Srguleng,ZHAO Qian-cheng,ZANG Sbu,JIN Feng-xie.Determination of Polysaccharide in Ophiopogon japonicus with 3,5-Dinitrosalicylic Acid Colorimeitry[J].Journal of Shenyang Agricultural University,2005,36(5):628-630.
Authors:WANG Hong-ying  QIAN Srguleng  ZHAO Qian-cheng  ZANG Sbu  JIN Feng-xie
Institution:WANG Hong-ying1,QIAN Srguleng1,ZHAO Qian-cheng2,ZANG Shu1,JIN Feng-xie1
Abstract:3,5-Dinitrosalicylic acid(DNS) colorimetry was used to determine the content of polysaccharide in Ophiopogon japonicus.The result showed that the content of polysaccharide in Ophiopogon japonicus was 40.4 mg.g-1 with RSD=2.48%(n=5);average recoveries of total sugar solution and monosaccharide solution in the test were 100.4% with RSD,0.17%(n=5) and 101.1% with RSD,0.62%(n=5) respectively.This method could be used to determine the contents of polysaccharide in Ophiopogon japonicus.
Keywords:Ophiopogon japonicus  polysaccharide  3  5-dinitrosalicylic acid
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