| 1株红树内生真菌Fusarium sp.代谢产物的研究 |
| |
| 引用本文： | 李文生,周丹丽,陆盈池,钟浩然,朱新伟,丁唯嘉,李春远. 1株红树内生真菌Fusarium sp.代谢产物的研究[J]. 华南农业大学学报, 2017, 38(3): 64-69. DOI: 10.7671/j.issn.1001-411X.2017.03.011 |
| |
| 作者姓名： | 李文生 周丹丽 陆盈池 钟浩然 朱新伟 丁唯嘉 李春远 |
| |
| 作者单位： | 华南农业大学 材料与能源学院,广东 广州,510642 |
| |
| 基金项目： | 国家自然科学基金（21102049）；广东省自然科学基金 (2015A030313405，9451064201003751)；广东省科技计划（2016A020222019）；教育部留学回国人员科研启动基金（［2015］311）；2017广州市科技计划科学研究一般项目（201707010342）；广东省大学生科技创新训练计划（201610564225，201510564196） |
| |
| 摘 要： | 【目的】研究红树林镰刀菌属真菌Fusarium sp.R5的代谢产物。【方法】采用硅胶柱层析分离纯化代谢产物,波谱技术鉴定结构,滤纸片扩散法测试抗植物病原真菌活性。【结果】分离鉴定5-羟甲基-2-呋喃甲醛(化合物1),(3R,4R)-顺-4-羟基蜂蜜曲菌素(化合物2),(3R,4R)-顺-4,7-二羟基蜂蜜曲菌素(化合物3),Clavatol(化合物4),酒渣碱(化合物5),麦角甾-4,6,8(14),22-四烯-3酮(化合物6),β-谷甾醇(化合物7)、3β-胆甾-5-烯-3-醇(化合物8)、丁二酸和顺丁烯二酸10个化合物。在250μg·m L~(-1)时,化合物5对香蕉炭疽菌Colletotrichum musae高度抗菌,化合物5和6对番茄枯萎菌F.oxysporum、小麦赤霉菌F.graminearum中度抗菌。【结论】从Fusarium属分离得到化合物1~8,其中,化合物5、6可作为相应农药抗菌先导化合物开展深入研究。
|
| 关 键 词： | 红树内生真菌 Fusariumsp. 代谢产物 抗真菌活性 |
| 收稿时间： | 2016-07-01 |
Study on the metabolites of a mangrove endophytic fungus Fusarium sp. |
| |
| LI Wensheng,ZHOU Danli,LU Yingchi,ZHONG Haoran,ZHU Xinwei,DING Weijia and LI Chunyuan. Study on the metabolites of a mangrove endophytic fungus Fusarium sp.[J]. JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY, 2017, 38(3): 64-69. DOI: 10.7671/j.issn.1001-411X.2017.03.011 |
| |
| Authors: | LI Wensheng ZHOU Danli LU Yingchi ZHONG Haoran ZHU Xinwei DING Weijia LI Chunyuan |
| |
| Affiliation: | College of Materials and Energy, South China Agricultural University,,College of Materials and Energy, South China Agricultural University,,College of Materials and Energy, South China Agricultural University,,College of Materials and Energy, South China Agricultural University,,College of Materials and Energy, South China Agricultural University,,College of Materials and Energy, South China Agricultural University, and College of Materials and Energy, South China Agricultural University, |
| |
| Abstract: | [Objective] To study the metabolites of a mangrove fungus Fusarium sp.R5.[Method]The fermented filtrate of R5 fungus was isolated by silica gel column chromatography.The structures of the metabolites were characterized by spectral analyses.The antifungal activities were investigated using the paper disc-agar diffusion method.[Result]Ten compounds were isolated and identified as 5-hydroxymethyl-2-furaldehyde (compound 1), (3R,4R)-cis-4-hydroxymellein (compound 2), (3R,4R)-cis-4,7-dihydroxymellein (compound 3), clavatol (compound 4), flazine (compound 5), ergosta-4, 6, 8(14), 22-tetraen-3-one (compound 6), β-sitosterol (compound 7), 3β-cholest-5-en-3-ol (compound 8), butanedioic acid and maleic acid.At the concentration of 250 μg·mL-1, compound 5 showed high inhibitory activity against Colletotrichum musae (Berk.&M.A.Curtis) Arx., compounds 5 and 6 showed moderate inhibitory activity against F.oxysporum and F.graminearum Schw.[Conclusion]Compounds 1~8 are isolated from Fusarium sp.and among them, compounds 5 and 6 could be used as the lead compounds in antifungal agents. |
| |
| Keywords: | mangrove endophytic fungus Fusarium sp. metabolite antifungal activity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《华南农业大学学报》浏览原始摘要信息 |
|
点击此处可从《华南农业大学学报》下载全文 |
|
