| 辣椒“三系”线粒体DNA的RAPD分析* |
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| 引用本文： | 邓明华,文锦芬,邹学校,朱海山,张竹青,赵凯.辣椒“三系”线粒体DNA的RAPD分析*[J].云南农业大学学报,2010,25(4):541-545. |
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| 作者姓名： | 邓明华 文锦芬 邹学校 朱海山 张竹青 赵凯 |
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| 作者单位： | 1.云南农业大学 园林园艺学院，云南 昆明 650201;2.中南大学 隆平分院，湖南 长沙 410125;3.昆明理工大学 现代农业工程学院，云南 昆明 650224;4.湖南省农业科学院 蔬菜研究所，湖南 长沙 410125 |
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| 基金项目： | 国家"863"计划，云南省"十一五"科技攻关项目，云南省应用基础研究项目，云南省教育厅项目 |
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| 摘 要： | 对辣椒胞质雄性不育系、保持系及恢复系线粒体DNA进行了RAPD分析，共使用500条随机引物，其中有67个引物在“三系”之间都得到了扩增产物，19条引物扩增结果在“三系”之间表现出了遗传多样性。在不育系与保持系的线粒体DNA之间也发现了明显的差异，在辣椒胞质不育系中获得特异片段9条，这些差异片段与辣椒雄性不育的关系还需进一步研究。
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| 关 键 词： | 辣椒 线粒体DNA RAPD 雄性不育 |
Analysis of mtDNA of Cytoplasmic Male Sterility (CMS) Line,Its Maintainer Line and Restorer Line of Pepper (Capsicum annuum L.) by RAPD |
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| DENG Ming-hua,WEN Jin-fen,ZOU Xue-xiao,ZHU Hai-shan,ZHANG Zhu-qing,ZHAO Kai.Analysis of mtDNA of Cytoplasmic Male Sterility (CMS) Line,Its Maintainer Line and Restorer Line of Pepper (Capsicum annuum L.) by RAPD[J].Journal of Yunnan Agricultural University,2010,25(4):541-545. |
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| Authors: | DENG Ming-hua WEN Jin-fen ZOU Xue-xiao ZHU Hai-shan ZHANG Zhu-qing ZHAO Kai |
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| Institution: | 1. College of Horticulture and Landscape, Yunnan Agricultural University, Kunming 650201, China;2. Branch of Longping, Central South University, Changsha 410125, China;3. Faculty of Modern Agricultural Engineering, Kunming University of Science and Technology, Kunming 650224, China;4. Institute of Vegetable Crops, Hunan Academy of Agricultural Sciences, Changsha 410125, China |
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| Abstract: | The mitochondrial DNA of cytoplasmic male sterility (CMS) line, its maintainer line and restorer line of pepper (Capsicum annuum L.) were studied by RAPD analysis. All of lines had obtained amplified products through 67 out of total 500 random primers. They emerged polymorphism in three lines through 19 primers. Some obvious differences in mitochondrial DNA were detected between CMS and its maintainer line, and 9 special bands in CMS line were found. The relationship of those different bands in CMS to cytoplasmic male sterility needs farther study. |
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| Keywords: | RAPD |
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