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两个水稻抗褐飞虱隐性基因的遗传分析与初步定位
引用本文:侯丽媛,于萍,徐群,袁筱萍,余汉勇,王一平,王彩红,万国,彭锁堂,魏兴华. 两个水稻抗褐飞虱隐性基因的遗传分析与初步定位[J]. 中国水稻科学, 2010, 24(4): 367-371. DOI: 10.3969/j.issn.1001-7216.2010.04.006
作者姓名:侯丽媛  于萍  徐群  袁筱萍  余汉勇  王一平  王彩红  万国  彭锁堂  魏兴华
作者单位:1. 中国水稻研究所,水稻生物学国家重点实验室,浙江,杭州310006;山西农业大学,农学院,山西,太谷030801
2. 中国水稻研究所,水稻生物学国家重点实验室,浙江,杭州310006
3. 山西农业大学,农学院,山西,太谷030801
基金项目:中央级公益性科研院所专项资金资助项目,农业部农业野生植物资源保护专项资助项目,浙江省农业重大科技项目 
摘    要:选用抗褐飞虱的海南普通野生稻渗入系WB01与感虫品种9311杂交,构建F2群体。采用141个具有多态性的SSR标记对303个F2:3株系进行分析,并应用MapMaker/EXP3.0和Windows QTL Cartographer2.0对水稻抗褐飞虱的数量性状基因座进行检测和遗传效应分析。共检测到2个抗性QTL,分别位于第4和第8染色体上,LOD值分别为2.92和3.15,贡献率分别为11.3%和14.9%。

关 键 词:褐飞虱  普通野生稻  抗性基因  数量性状基因座  基因定位
收稿时间:1900-01-01;

Genetic Analysis and Preliminary Mapping of Two Recessive Resistance Genes in Rice to Brown Planthopper, Nilaparvata lugens
HOU Li-yuan,YU Ping,XU Qun,YUAN Xiao-ping,YU Han-yong,WANG Yi-ping,WANG Cai-hong,WAN Guo,PENG Suo-tang,WEI Xing-hua. Genetic Analysis and Preliminary Mapping of Two Recessive Resistance Genes in Rice to Brown Planthopper, Nilaparvata lugens[J]. Chinese Journal of Rice Science, 2010, 24(4): 367-371. DOI: 10.3969/j.issn.1001-7216.2010.04.006
Authors:HOU Li-yuan  YU Ping  XU Qun  YUAN Xiao-ping  YU Han-yong  WANG Yi-ping  WANG Cai-hong  WAN Guo  PENG Suo-tang  WEI Xing-hua
Affiliation:State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; College of Agronomy, Shanxi Agricultural University, Taigu 030801, China; * Corresponding author, E-mail: xwei@mail.hz.zj.cn
Abstract:An F2 population was derived from the cross of the brown planthopper resistant line WB01, an introgression line of common wild rice (Oryza rufipogon Griff.) from Hainan Province, China and the susceptible indica cultivar 9311. The population with 303 lines was genotyped using 141 simple sequence repeat (SSR) markers and used for gene mapping. Two softwares, MapMaker/Exp 3.0 and Windows QTL Cartographer 2.0 were applied to detect QTLs. Totally, two QTLs on chromosomes 4 and 8 were detected. Each of these QTLs individually explained 11.3% and 14.9% of the phenotypic variations, respectively.
Keywords:brown planthopper  Oryza rufipogon  resistance gene  quantitative trait locus  gene mapping
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