钝裂银莲花不同居群遗传多样性的ISSR分析

本研究采用ISSR分子标记技术分析了甘肃省4个地区(合作、碌曲、阿万仓和阿孜)钝裂银莲花的11个居群的遗传多样性水平和遗传结构。结果表明,13个引物共扩增出701条带,其中392条具有多态性,多态位点百分率为55.92%。在物种水平上,Shannon多样性指数(I)为0.372,Nei基因多样性指数(H)为0.250;Nei的基因分化系数Gst和AMOVA分析表明钝裂银莲花居群遗传分化大部分存在于居群间。在居群水平上,I为0.183,H为0.114,可见钝裂银莲花居群具有较高的遗传多样性。依据Nei的遗传距离对不同居群进行UPGMA聚类,聚类结果为合作居群为一类,碌曲和阿万仓居群为一类,阿孜为一类。居群遗传多样性与环境因子间的相关性分析表明,遗传多样性水平与海拔、含水量、有效磷和经纬度之间没有相关性,而与降水量之间显著负相关。

Analysis of genetic diversity in Anemone obtusiloba populations with ISSR markers

Eleven species of Anemone obtusiloba collected from 4 areas of Gansu were analysed by inter single sequence repeats (ISSR) to elucidate the inherit diversity level and structure. Seven hundred and one bands were amplified by 13 primers and 392 bands were polymorphic, a polymorphic locus percentage (P) of 55.92%. The Shannon index (I) was 0.372 and Nei’s gene diversity (H) was 0.250. Based on Nei’s Gst value and analysis of molecular variance (AMOVA), showed that species inheritance of differentiation of A. obtusiloba existed mainly. In inter-populations: I was 0.183 and H was 0.114 at the population level. These data indicated that there were high inherent diversities in the population of A. obtusiloba. Using UPGMA, three populations were clustered: HZ, LQ and AWC, and AZ. Further analysis showed that there was no correlation between population genetic diversity level and altitude, water, extractable phosphorous or longitude and latitude, but there was a significantly negative correlation with precipitation.