| 小鹅瘟免疫荧光抗体的制备及检测方法研究 |
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| 引用本文： | 吕雪峰,李忠勋,李志慧,吉凤涛,苏双. 小鹅瘟免疫荧光抗体的制备及检测方法研究[J]. 中国动物保健, 2012, 0(2): 13-15 |
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| 作者姓名： | 吕雪峰 李忠勋 李志慧 吉凤涛 苏双 |
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| 作者单位： | [1]吉林省畜牧兽医科学研究院,吉林长春130062 [2]磐石市吉昌镇畜牧站,吉林磐石132300 [3]吉林大学超硬材料国家重点实验室,吉林长春130012 |
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| 基金项目： | 家畜疫病病原生物学国家重点实验室开放基金资助（SKLVEB2010KFKT007）; 吉林省科技发展计划资助项目（20090162） |
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| 摘 要： | 本试验建立有效的小鹅瘟检测方法。通过利用鹅细小病毒免疫实验鹅,提取抗体（IgG）,并利用异硫氰酸荧光素（FITC）染色。利用制备的荧光抗体来对小鹅瘟病例进行直接免疫荧光诊断。经实验,制备出的鹅IgG荧光抗体敏感性较高、特异性较强,并建立较为高效的小鹅瘟直接免疫荧光检测方法。
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| 关 键 词： | 鹅 抗体 免疫荧光 诊断 |
Preparation of Gosling plague Immunofluorescence Antibody and Study of Diagnosis Method |
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| Lv Xuefeng,Li Zhongxun,Li Zhihui,Ji Fengtao,Su Shuang. Preparation of Gosling plague Immunofluorescence Antibody and Study of Diagnosis Method[J]. China Animal Health, 2012, 0(2): 13-15 |
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| Authors: | Lv Xuefeng Li Zhongxun Li Zhihui Ji Fengtao Su Shuang |
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| Affiliation: | 1. Veterinary Research Science Institute of Jilin Province, changchun Jinlin 130062; 2. Jichang Veterinary Medicine and Feed Supervision Institute of Panshi, Panshi Jilin ! 32300; 3. National Laboratory of Superhard Materials Jilin University, Changchun Jilin 130012 ) |
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| Abstract: | To establish an effective method for diagnosis of Gosling plague. We immuned goose and picked up goose anti-GP IgG, marked with FITC and acquired immunofluorescence antibody which directly diagnosis Gosling plague. The immunofluorescence antibodywas examined by absorb experiment and specific inhibit experiment. The result showed that the method of immunofluorescence antibody diagnosis of Gosling plague was higher, specific and sensitive. |
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| Keywords: | goose antibody immunofluorescence diagnosis |
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