| Cloning and Sequence Analysis of the gp41 Gene of Clanis bilineata Nuclear Polyhedrosis Virus |
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| 作者姓名： | ZHU Shan-ying WANG Wen-bing ZHU Jiang |
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| 作者单位： | [1]Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, P.R.China [2]College of Life Sciences, Suzhou University, Suzhou 215006, P.R.China |
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| 基金项目： | 国家重点基础研究发展计划(973计划);江苏大学校科研和教改项目 |
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| 摘 要： | Clanis bilineata Nucleo Polyhedro Virus （CbNPV） was purified from Clanis bilineata larva. To obtain the molecular information of the virus, the genomic DNA of CbNPV was extracted, and a DNA fragment library of the virus was constructed using shotgun. The positive clones were then sequenced and analyzed. An open-reading frame （ORF） that has high identity with the gp41 gene of most NPVs was found in the library. The gp41 gene of CbNPV is 933 base pair long and encodes a protein of 310 amino acids. The result of the amino acid sequence analysis showed that the CbNPV gp41 has 53-61 and 56-73% identities with Group 1 and II NPVs gp41 proteins, respectively. The result indicates that the isolated CbNPV is a novel baculovirus, and the CbNPV shares a much closer relationship Ⅱ NPVs.
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| 关 键 词： | 克隆技术 基因 序列 幼体 CbNPV 病毒 |
Cloning and Sequence Analysis of the gp41 Gene of Clanis bilineata Nuclear Polyhedrosis Virus |
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| ZHU Shan-ying WANG Wen-bing ZHU Jiang.Cloning and Sequence Analysis of the gp41 Gene of Clanis bilineata Nuclear Polyhedrosis Virus[J].Agricultural Sciences in China,2006,5(10):787-792. |
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| Authors: | ZHU Shan-ying WANG Wen-bing ZHU Jiang |
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| Institution: | 1. Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, P.R. China
2. College of Life Sciences, Suzhou University, Suzhou 215006, P.R. China |
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| Abstract: | Clanis bilineata Nucleo Polyhedro Virus (CbNPV) was purified from Clanis bilineata larva. To obtain the molecular information of the virus, the genomic DNA of CbNPV was extracted, and a DNA fragment library of the virus was constructed using shotgun. The positive clones were then sequenced and analyzed. An open-reading frame (ORF) that was high identity with the gp41 gene of most NPVs was found in the library. The gp41 gene of CbNPV is 933 base pair long and encodes a protein of 310 amino acids. The result of the amino acid sequence analysis showed that the CbNPV gp41 has 53–61 and 56–73% identities with Group I and II NPVs gp41 proteins, respectively. The result indicates that the isolated CbNPV is a novel baculovirus, and the CbNPV shares a much closer relationship with Group II NPVs. |
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| Keywords: | Clanis bilineata Nucleo Polyhedro Virus gp41 gene sequence analysis |
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