| 攀西地区葛藤根瘤菌的RFLP遗传多样性 |
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| 引用本文： | 谢徽,张小平,K.Lindstrom.攀西地区葛藤根瘤菌的RFLP遗传多样性[J].四川农业大学学报,2009,27(3). |
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| 作者姓名： | 谢徽 张小平 K.Lindstrom |
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| 作者单位： | 1. 四川农业大学,资源环境学院,四川,雅安,62500
2. 赫尔辛基大学,应用化学与微生物学系,芬兰,赫尔辛基,00014 |
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| 摘 要： | 利用16S rDNA PCR-RFLP和16-23S IGS PCR-RFLP技术对分离自四川攀枝花的43株葛藤根瘤菌进行了遗传多样性研究。供试菌株的16S rDNA用HaeⅢ、MspⅠ、HinfⅠ和TaqⅠ酶切后具有16种遗传图谱类型。16S-23S rDNA IGS PCR-RFLP分析结果表明,在53%的相似性水平上,供试菌株与参比菌株分为两个分支,在81%的相似水平上所有菌株分为13个群。
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| 关 键 词： | 根瘤菌 葛藤 16S rDNA PCR-RFLP 16-23S IGS PCR-RFLP 遗传多样性 |
RFLP Genetic Diversity of Rhizobia Isolated from Pueraria lobata in Panxi |
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| K.Lindstrom.RFLP Genetic Diversity of Rhizobia Isolated from Pueraria lobata in Panxi[J].Journal of Sichuan Agricultural University,2009,27(3). |
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| Authors: | KLindstrom |
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| Abstract: | Forty-three Rhizobia strains were isolated and analyzed by using 16S rDNA and 16-23S rDNA intergernic spacer(IGS) PCR-RFLP,and the PCR fragments were digested by restriction endonucleases including HaeⅢ,MspⅠ,HinfⅠ and TaqⅠ.The results showed that 16S rDNA PCR-RFLP divided all the strains into 16 genotypes,and the results of 16-23S rDNA PCR-RFLP showed that all the strains clustered 2 geno-branches at the similarity of 53%,and were divided into 13 groups at the similarity of 81%. |
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| Keywords: | 16S rDNA PCR-RFLP 16-23S IGS PCR-RFLP |
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