核用银杏品种遗传关系的AFLP分析

采用AFLP技术对34份核用银杏品种进行遗传多样性和亲缘关系分析。结果表明：8对AFLP引物组合共扩增出1 337条谱带，多态带百分率为99.63%；品种间的遗传相似系数在0.2915 ~ 0.6812之间，平均值为0.4911；在相似系数为0.50时供试品种可分为4大类；依据AFLP结果建立了核用银杏品种指纹图谱。核用银杏4个类型的Nei’s遗传多样性指数范围为0.1392 ~ 0.2014，总体为0.2112，Shannon’s信息指数范围为0.2138 ~ 0.3165，总体为0.3417；4个类型品种间的遗传分化系数为0.0871，基因流为5.4230。结合特异位点、相似系数、聚类结果等筛选出8份核用银杏优质品种。

Genetic Relationship of Stone-used Ginkgo biloba Cultivars Based on AFLP Markers

The genetic relationship of 34 stone-used Ginkgo biloba L. cultivars were assessed by AFLP markers. A total of 1 337 bands were amplified with 8 pairs of primers，the percentage of polymorphic loci was 99.63%. The genetic similarity coefficient among 34 stone-used G. biloba L. cultivars ranged from 0.2915 to 0.6812 with an average of 0.4911，and all cultivars were divided into 4 groups at 0.50 of the similarity coefficient. The fingerprint of G. biloba L. cultivars were established based on AFLP results. Nei’s gene diversity index ranged from 0.1392 to 0.2014 for 4 types of stone-used G. biloba L. cultivars，with the total value of 0.2112. Shannon’s information index varied from 0.2138 to 0.3165，with the total value of 0.3417. The genetic differentiation coefficient for 4 types of stone-used G. biloba L. cultivars was 0.0871 and genetic flow was 5.4230. Eight cultivars were recognized relatively more distinctive among 34 stone-used G. biloba L. cultivars.