低温锻炼对两种绣线菊光合作用蛋白及光合能力的影响

以抗寒力不同的毛果绣线菊与金山绣线菊为试验材料，测定低温下光合特性的变化，利用蛋白质组学核心技术建立两种绣线菊低温下光合作用蛋白的表达图谱，并鉴定光合作用蛋白种类，探讨两种绣线菊对低温锻炼的响应机制。结果表明：低温下两种绣线菊的光合能力下降，利用弱光的能力增强，利用强光的能力降低，抗寒能力较强的毛果绣线菊利用弱光能力强于抗寒能力较弱的金山绣线菊。在不同低温水平下分别鉴定出涉及能量代谢与运输的光合作用蛋白共17种，低温下金山绣线菊中参与卡尔文循环、三羧酸循环、磷酸戊糖途径和光合磷酸化的酶显著上调和下调表达，毛果绣线菊中仅参与卡尔文循环的酶和参与PSⅡ光合电子传递的蛋白明显上调和下调表达；低温使两种绣线菊光合作用均受到影响，金山绣线菊光合作用蛋白在低温下的变化更为显著。

Effects of Low Temperature on Photosynthetic Proteins and Photosynthetic Capacities of Two Species of Spiraea

Spiraea trichocarpa Nakai and S. bumalda‘Gold Mound’，which have different cold resistance ablilty were used as experimental materials，for measuring the change of photosynthetic characteristics，establishing the different expression protein patterns of two species Spiraea in low temperature by the core technology of proteomics and then identifying the species of photosynthetic proteins，investigating the respond mechanism of two species Spiraea to low temperature treatments. The results showed that the photosynthetic capacities of two Spiraea species decreased in low temperature，but their capacities of using weak light increased and the capacities of using strong light decreased，S. trichocarpa Nakai with stronger cold resistance showed a better ability of using weak light than that of S. bumalda‘Gold Mound’with the weaker cold resistance. Seventeen kinds of photosynthetic proteins involved in energy metabolism and transport were identified seperately under different low temperature，the enzymes involved in Calvin cycle，TCA cycle，the pentose phosphate pathway and photophosphorylation of S. bumalda‘Gold Mound’showed obvious up and down regulation，while in S. trichocarpa Nakai only the enzymes involved in Calvin cycle and the proteins of photosynthetic electron transportation in PSⅡ showed obvious up and down regulation in low temperature；The photosynthesis of two species Spiraea were affected by low temperature，the change of the photosynthetic proteins in S. bumalda‘Gold Mound’were more significant.