短短芽孢杆菌JK-2的GFP标记及其抑菌作用

本文采用gfp基因标记枯萎病生防菌短短芽孢杆菌Brevibacillus brevis菌株JK-2。标记菌株菌落圆形,菌体短杆状,与原始菌株相同;荧光显微观察发现菌落和菌体发绿色荧光。标记菌株起始期生长缓慢,进入对数生长期后生长迅速。在无选择压力条件下连续转接15次,GFP标记仅丢失11.7%。gfp标记菌株对不同植物枯萎病菌具有很强的抑菌能力,与野生型菌株相当。

GFP-Tagging and Antagonistic Activity of the Engineered Strains of Brevibacillus brevis JK-2

The green fluorescent protein gene（gfp） was transferred into the biocontrol bacterium,Brevibacillus brevis JK-2.The colony and cell morphology of the gfp tagged strain were the same as those of the wild type.Fluorescent microscope observation showed that the green fluorescence was detectable in colonies and cells of the gfp tagged strain.The transformants grew more slowly during the initial phase and then faster in the log-phase compared with the wild type.The transformed strain could express GFP stably without antibiotic pressure,the GFP marker only lost 11.7% after transference of culture 15 times continuously.Inhibition activity of the gfp tagged strain against different specialized forms of Fusarium oxysporum was almost the same as that of the wild type.