生防菌枯草芽孢杆菌BS-5在3L发酵罐中的发酵工艺优化

为了提高生防菌枯草芽孢杆菌BS-5液体发酵的生物量，在3L发酵罐中进行了分批发酵和补料发酵试验。在分批发酵中，将氮源物质豆粕浓度由2％提高到4％之后，生物量由4.8×10^9cfu／ml提高到8.2×10^9cfu／ml。在分批补料发酵中，采取分批多次补糖策略。初糖浓度为20g／L，当糖浓度在10g／L以下时，补加葡萄糖（每次补加量为20g／L），共补加两次，生物量提高到2.8×10^10cfu／ml。

Optimization of Fermentation Technology of Biocontrol Strain Bacillus subtilis BS-5 in 3-Liter Fermentor

In order to increase the biomass of Bacillus subtilis, batch fermentation and fed-batch fermentation experiments were performed in 3 liter fermentor. Results showed that the biomass was increased from 4.8×10^9 cfu/ml to 8.2×10^9 cfu/ml while the soy bean powder concentration was increased from 2% to 4% at batch fermentation. In the course of fed-batch culture, three modes of glucose feeding strategy were employed, of which the best mode was that the suitable initial concentration of glucose was 20 g/L, when the glucose concentration in broth was below 10 g/L, condensed glucose was fed into the broth twice, the supplying quantity was 20 g/L every time, the biomass was up to 2.8×10^10 cfu/ml.