| 一株毒杀松材线虫的解淀粉芽孢杆菌活性物质的基本性质 |
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| 引用本文： | 朱丽梅,吴小芹,陈江天,徐旭凌.一株毒杀松材线虫的解淀粉芽孢杆菌活性物质的基本性质[J].中国生物防治,2009,25(4):359-363. |
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| 作者姓名： | 朱丽梅 吴小芹 陈江天 徐旭凌 |
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| 作者单位： | 1. 南京林业大学森林资源与环境学院/江苏省有害生物预防与控制重点实验室,南京,210037;金陵科技学院园艺学院
2. 南京林业大学森林资源与环境学院/江苏省有害生物预防与控制重点实验室,南京,210037 |
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| 基金项目： | 国家林业局"948"项目 |
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| 摘 要： | 从马尾松林中筛选出的解淀粉芽孢杆菌Bacillus amyloliquefaciens JK-JS3菌株对松材线虫Bursaphelenchus xylophilus具有较高杀线活性。本试验对JK-JS3菌株分泌的杀线活性物质的基本性质进行了研究。结果表明：该菌株产生的杀线组分是可随水分一同蒸发的非蛋白质类物质;其吸收峰在190nm到200nm之间;常温25℃和冷藏4℃下保存60d仍能保持杀线活性;不同温度和pH条件对其杀线组分的活性有影响,加热会降低其活性,碱性条件下具有较高的杀线活性;石油醚、氯仿、乙酸乙酯等有机溶剂不能将其活性物质从粗提液中提取。
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| 关 键 词： | 松材线虫 解淀粉芽孢杆菌 杀线活性 基本性质 |
Elementary Properties of the Nematicidal Crude Toxin Produced by Bacillus amyloliquefaciens JK-JS3 |
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| ZHU Li-mei
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WU Xiao-qin
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CHEN Jiang-tian
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XU Xu-ling.Elementary Properties of the Nematicidal Crude Toxin Produced by Bacillus amyloliquefaciens JK-JS3[J].Chinese Journal of Biological Control,2009,25(4):359-363. |
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| Authors: | ZHU Li-mei WU Xiao-qin CHEN Jiang-tian XU Xu-ling |
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| Institution: | ZHU Li-mei, WU Xiao-qin , CHEN Jiang-tian , XU Xu-ling ( 1. Nanjing Forestry University, Key Laboratory of the Pest Prevention and Control of the Jiangsu Province, Nanjing 210037, China;2. College of Gardening of Jinling Institute of Technology, Nanjing 210038, China) |
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| Abstract: | Bacillus amyloliquefaciens JK-JS3, isolated from the leaf of the Pinus massoniana in Mount Zijin, has high nematicidal activity to Bursaphelenchus xylophilus. In order to isolate and purify the nematicidal components, the elementary characteristics of the nematicidal crude toxin have been studied in our experiment. The results indicated that the nematicidal toxin is not a protein which can be evaporated with the water and its maximum optical absorption is from 190 to 220 nm. The nematicidal activity of the toxin can sustain for a long period at 25℃ and 4℃. High temperature and pH condition can affect its nematicidal activity. It has strong nematicidal activity at the alkalinity condition. The nematicidal components can not be dissolved in the organic solvents such as petroleum ether, chloroform and ethyl acetate. |
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| Keywords: | Bursaphelenchus xylophilus Bacillus amyloliquefaciens nematicidal activity elementary property |
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