| 风信子DFR基因全长cDNA的克隆及序列分析 |
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| 引用本文： | 祁银燕,刘雅莉,李 莉,王跃进.风信子DFR基因全长cDNA的克隆及序列分析[J].中国农学通报,2009,25(24):62-67. |
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| 作者姓名： | 祁银燕 刘雅莉 李 莉 王跃进 |
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| 作者单位： | 西北农林科技大学园艺学院;农业部西北园艺植物种质资源利用重点开放实验室, 陕西省农业分子生物学重点实验室,陕西杨凌,712100 |
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| 基金项目： | 国家自然科学基金（30871734） |
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| 摘 要： | 以蓝色风信子完全露出蓝色的花蕾为材料,利用RT-PCR和RACE技术，获得风信子DFR基因的全长cDNA。该cDNA全长1252 bp ,开放阅读框为1098 bp ,编码366个氨基酸。 Blast搜索结果显示,风信子DFR基因核苷酸序列与其它植物已报道的DFR基因具有66 %～77 % 的相似性,氨基酸序列有62 %～73 %的相似性。聚类分析表明, 最先与风信子聚类合并的是鸢尾，其次与其它单子叶植物聚类，最后与双子叶植物聚类。
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| 关 键 词： | 耐甲氧西林葡萄球菌 耐甲氧西林葡萄球菌 mecA基因 PCR 核酸探针 |
| 收稿时间： | 2009-08-24 |
| 修稿时间： | 2009-09-30 |
Cloning and Sequencing Full-length cDNA of DFR gene in Hyacinthus |
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| Qi Yinyan,Liu Yali,Li Li,Wang Yuejin.Cloning and Sequencing Full-length cDNA of DFR gene in Hyacinthus[J].Chinese Agricultural Science Bulletin,2009,25(24):62-67. |
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| Authors: | Qi Yinyan Liu Yali Li Li Wang Yuejin |
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| Institution: | (College of Horticulture, Northwest A ＆ F University; Key Laboratory of Horticultural Plant Germplasm Resources Utilization Northwest of China, Ministry of Agriculture, Key Laboratory of Agriculture Molecular Biology of Shaanxi Province, Yangling Shaanxi 712100) |
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| Abstract: | A total length DFR gene was cloned with RT-PCR and RACE technology from flower bud with blue petals of Hyacinthus orientalis L.. Hyacinthus DFR cDNA was 1252 bp in length.Within the f ull-length cDNA , a clear open reading frame (ORF) was 1098 nucleotides ,coding 366 amino acids. The result s of homologous analysis in GenBank demonstrated that the sequence had 66 %～77 % identity on the nucleotide sequence and 62 %～73% identity on the deduced amino acid sequence. The results of phylogenetic analysis showed that DFR from Hyacinthus orientalis and from Iris x hollandica clustered together firstly ,and then came those from other monocotyledon, dicotyledon came subsequently. |
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| Keywords: | DFRzz cDNA cloningzz Hyacinthuszz genezz |
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