适于杨树功能基因组研究的T-DNA激活标签构建

杨树(Populus spp.)分布遍及北半球,具有广泛的经济价值和生态价值.由于杨树具有相对较小的基因组(约500 Mb)、紧凑的基因组结构(n=19)及较为成熟的遗传转化方法,使其成为木本植物研究的模式树种种[1～4].

Construction of T-DNA Activation Tagging for Populus Functional Genomics

With the completion of the Populus genome sequence in 2006, the study on functional genomics in Populus has become a major task. Establishment of Populus mutant population is an essential approach for forestry functional genomics study. The activation tagging is a promising and powerful method to construct mutant library of Pop- ulus and to further discover new gene and elucidate its function now. A novel T-DNA activation tagging pCAS05 was constructed for forest functional genomics in this study. We could select conveniently and easily transgenic plants by spraying Basta because bar gene is constructed in this vector as a selection marker. Moreover, we could also construct a large scale mutant library in Populus by this activation tagging because of effectively selection of bar gene.