| 荧光原位PCR定位动物精子介导转移的外源基因 |
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| 引用本文： | 武坚,刘明军,李文蓉,郭志勤,陈东,张曼夫. 荧光原位PCR定位动物精子介导转移的外源基因[J]. 西北农业学报, 2000, 9(1): 10-13 |
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| 作者姓名： | 武坚 刘明军 李文蓉 郭志勤 陈东 张曼夫 |
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| 作者单位： | 1. 农业部畜牧兽医生物技术重点开放实验室,乌鲁木齐,830000
2. 中国农业大学生物学院,北京,100094 |
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| 摘 要： | 用荧光原位PCR对经体外介导外源基因转移方法处理的家畜精子进行检测。结果证实，牛，山羊和绵羊的精子在一定条件下能够携带所转移的外源基因，其平均转移效率分别为：绵羊27.12%，牛31.16%，山羊30.57%，同时发现外源基因已进入精子细胞内并位于核及其外围区域。
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| 关 键 词： | 动物精子 外源基因 精子介导 基因转移 荧光原位PCR 精子载体法 |
| 文章编号： | 1004-1389(2000)01-0010-04 |
| 收稿时间： | 1999-08-03 |
| 修稿时间： | 1999-08-03 |
Sperms-mediated Exogenous DNA Located by Fluoresent in Situ PCR Technique |
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| WU Jian,LIU Mingjun,LI Wenrong,GUO Zhiqin,CHEN Dong and ZHANG Manfu. Sperms-mediated Exogenous DNA Located by Fluoresent in Situ PCR Technique[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2000, 9(1): 10-13 |
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| Authors: | WU Jian LIU Mingjun LI Wenrong GUO Zhiqin CHEN Dong ZHANG Manfu |
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| Affiliation: | The Key Laboratory of Animal and Veterinary Biotechnology of Ministry of Agriculture, Urumqi 830000;The Key Laboratory of Animal and Veterinary Biotechnology of Ministry of Agriculture, Urumqi 830000;The Key Laboratory of Animal and Veterinary Biotechnology of Ministry of Agriculture, Urumqi 830000;The Key Laboratory of Animal and Veterinary Biotechnology of Ministry of Agriculture, Urumqi 830000;College of Biological Science, China Agricultural University, Beijing 100094;College of Biological Science, China Agricultural University, Beijing 100094 |
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| Abstract: | In order to located the exogenous DNA molecule intosperm cells,we used fluo rescent in situ PCR technique to determinate the efficiency of sperms of the livestock as vector mediated the exogenous DNA transfer into sperm cells.The results showed that the sperm cells are able to take up exogenous DNA molecule in cattle,sheep and goat at experiment conditions,internalize into sperm''s nuclei,and locateat its per ipheral to the nucleus region.The average efficiency for DNA binding is 27.12% in sheep,31.16% in cattle and 30.57% in goat. |
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| Keywords: | Sperm mediated Gene transformation Fluorescent in situ PCR(PRINS) |
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