Cryopreservation of dormant orthodox seeds of forest trees: mazzard cherry (Prunus avium L.) |
| |
Authors: | Paweł Chmielarz |
| |
Affiliation: | 1. Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035, Kórnik, Poland
|
| |
Abstract: | ? The sensitivity of dormant seeds of mazzard cherry (Prunus avium L.) of Polish provenances to extreme desiccation and/or the ultra-low temperature of liquid nitrogen, LN (?196 °C), was investigated to identify the optimum seed water content (w.c.) at this temperature. ? Germination and seedling emergence tests in this study showed that there is no critical seed w.c. for deeply desiccated seeds (stone w.c. 1.6%), although such desiccated seeds were sensitive to the temperature of LN in the seedling emergence test. For seeds frozen in LN, the highest germinability was observed at w.c. of 9.0–16.9%, but seedling emergence was then significantly lower than in nonfrozen seeds. The 2-year of storage in LN of seeds desiccated to 7.8% w.c. did not decrease germinability after thawing, in comparison with 2-year of storage at ?3 °C. Storage in LN showed that if seeds were stored after breaking of their dormancy, germinability after storage was lower because of the necessity of seed desiccation to the lower level (~ 8%) of w.c. after stratification (before storage). Secondary dormancy was induced in seeds desiccated after stratification. ? The results of this study demonstrate the potential for long-term cryopreservation of mazzard cherry seeds in forest gene banks. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|