| 翘嘴鳜和翘嘴红鲌血细胞DNA含量测定 |
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| 引用本文： | 许晓军,杜建明,张海琪,张晓辉.翘嘴鳜和翘嘴红鲌血细胞DNA含量测定[J].浙江农业学报,2012,24(3):392-395. |
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| 作者姓名： | 许晓军 杜建明 张海琪 张晓辉 |
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| 作者单位： | 浙江省水产技术推广总站,浙江杭州,310012 |
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| 摘 要： | 以翘嘴鳜(Siniperca chuatsi)和翘嘴红鲌(Erythroculter ilishaeformis)的血细胞为材料,以鸡血细胞为DNA标准(2.5 pg/2C),利用流式细胞仪(Beckman Coulter Quanta SC)分别采用内标法和外标法测定其细胞核DNA含量。结果表明,翘嘴鳜和翘嘴红鲌的血细胞DNA含量分别为鸡血细胞的0.69倍和0.87倍,换算成绝对含量分别为1.74 pg/2C和2.18 pg/2C。
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| 关 键 词： | 翘嘴鳜 翘嘴红鲌 DNA含量 流式细胞仪 |
Determination of cellular DNA content of Siniperca chuatsi and Erythroculter ilishaeformis |
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| XU Xiao-jun
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DU Jian-ming
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ZHANG Hai-qin
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ZHANG Xiao-hui.Determination of cellular DNA content of Siniperca chuatsi and Erythroculter ilishaeformis[J].Acta Agriculturae Zhejiangensis,2012,24(3):392-395. |
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| Authors: | XU Xiao-jun DU Jian-ming ZHANG Hai-qin ZHANG Xiao-hui |
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| Institution: | (Zhejiang Fishery Technical Extention Center,Hangzhou 310012,China) |
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| Abstract: | The cellular DNA contents of Siniperca chuatsi and Erythroculter ilishaeformis were studied using flow cytometry with Gallus domestics red blood cells as an internal / external standard.The results showed that the DNA contents of Siniperca chuatsi and Erythroculter ilishaeformis were 0.69 and 0.87 times of Gallus domestics,respectively.The absolute DNA contents were 1.74 pg/2C and 2.18 pg/2C,respectively. |
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| Keywords: | Siniperca chuatsi Erythroculter ilishaeformis DNA content flow cytometry |
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