Purified Culture Systems for Bovine Oviductal Stromal Cells |
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Authors: | Yuki YAMAMOTO Yoshihiko KOBAYASHI Kiyoshi OKUDA |
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Institution: | 1) Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan |
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Abstract: | Isolated stromal cells from the ampullary and isthmic parts of bovine oviductal tissues
were cultured in monolayer and spheroid (cell aggregate) systems. Prostaglandin F2α (PGF)
plays a crucial role in oviductal contraction and is produced by oviductal epithelial
cells in cattle. Since stromal cells of many organs produce PGF, PGF production by bovine
oviductal stromal cells was investigated. After PGF synthesis was confirmed, the utility
of isolation and culture methods for oviductal stromal cells was evaluated by PGF
production in the present study. The homogeneity of the cells was > 99%. PGF production
of the cells was increased by tumor necrosis factor-α. The stromal cells aggregated and
formed a spheroid by the treatments with several reagents. PGF production was higher in
the spheroid culture than in the monolayer culture. The isolation and culture methods
described here will facilitate studies of the physiological function of bovine oviductal
stromal cells. |
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Keywords: | Cell culture Cell-to-cell interaction Cow Oviductal stromal cell Spheroid |
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