|
|||||
|
|
| 夏蜡梅组织培养试验初报 | |
| 引用本文： | 邵果园,蔡荣荣,王力超,梁国鲁. 夏蜡梅组织培养试验初报[J]. 浙江林业科技, 2006, 26(5): 28-30 |
| 作者姓名： | 邵果园 蔡荣荣 王力超 梁国鲁 |
| 作者单位： | 1. 浙江林学院,林业与生物技术学院,浙江,临安,311300;西南大学,园艺园林学院,重庆,400716 2. 浙江林学院,林业与生物技术学院,浙江,临安,311300 3. 西南大学,园艺园林学院,重庆,400716 |
| 摘 要： | 以夏蜡梅茎段为外植体，进行夏蜡梅组培试验，结果表明：外植体茎段的最佳消毒方式是先用70％酒精消毒10—15s，再用0．1％HgCl溶液浸泡3—5min；继代增殖培养的最佳培养基为MS＋6-BA2．0mg／L＋NAA0．1mg／L＋蔗糖30g/L，增殖系数4．03，茎芽生长粗壮；最佳生根培养基为I／2MS＋IBA0．4mg/L。 |
| 关 键 词： | 夏蜡梅 组织培养 增殖 生根 |
| 文章编号： | 1001-3776(2006)05-0028-03 |
| 收稿时间： | 2006-08-20 |
| 修稿时间： | 2006-08-20 |
Study on Tissue Culture of Calycanthus chinensis |
|
| SHAO Guo-yuan,CAI Rong-rong,WANG Li-chao,LIANG Guo-lu. Study on Tissue Culture of Calycanthus chinensis[J]. Journal of Zhejiang Forestry Science and Technology, 2006, 26(5): 28-30 | |
| Authors: | SHAO Guo-yuan CAI Rong-rong WANG Li-chao LIANG Guo-lu |
| Abstract: | Experiment on tissue culture of Calycanthus chinensis by using stems as explant showed that the optimal sterilizing method was dipping the stems in 70% alcohol for 10 - 15 seconds and 0.1% Hgcl for 3 - 4 minutes. The optimum medium for multiplication was MS + 6-BA2.0mg/L + NAA0.1mg/L + sugar 30 g/L, and the rate of regeneration reached 4.03 with the IBA concentration of 0.4 mg/L in 1/2MS. |
| Keywords: | Calycanthus chinensis tissue culture multiplication rooting |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |