| MEV、ADV和CAV三种病毒多重PCR检测方法的建立 |
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| 引用本文： | 罗彬,易立,王建科,程世鹏.MEV、ADV和CAV三种病毒多重PCR检测方法的建立[J].动物医学进展,2009,30(2). |
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| 作者姓名： | 罗彬 易立 王建科 程世鹏 |
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| 作者单位： | 中国农业科学院特产研究所,吉林吉林,132109 |
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| 基金项目： | 吉林省科技发展计划项目 |
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| 摘 要： | 建立一种同时检测貂肠炎病毒(MEV)、貂阿留申病毒(ADV)和犬腺病毒(CAV)的多重PCR诊断方法.引用已有的CAV引物,并根据GenBank发表的MEV、ADV序列保守区域设计特异性引物进行PCR扩增,可同时得到扩增长度为795(MEV)、451(ADV)、1 019 bp(CAV)3奈特异性片段,对猪细小病毒(PPV),犬瘟热病毒(CDV)进行PCR检测结果为阴性.各种模板、引物之间相互不构成干扰.敏感性试验证明,可以检测到模板中MEV 101.5 TCID50和CAV 100.5 TCID50的病毒含量,对ADV检测的敏感性更高.
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| 关 键 词： | 多重PCR 貂肠炎病毒 貂阿留申病毒 犬腺病毒 |
Establishment of a Multiplex PCR for Detection of MEV,ADV and CAV |
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| LUO Bin,YI Li,WANG Jian-ke,CHENG Shi-peng.Establishment of a Multiplex PCR for Detection of MEV,ADV and CAV[J].Progress In Veterinary Medicine,2009,30(2). |
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| Authors: | LUO Bin YI Li WANG Jian-ke CHENG Shi-peng |
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| Institution: | Institute of Special Wild Economic Animal and Plant Science;CAAS;Jilin;132109;China |
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| Abstract: | A novel multiplex PCR(mPCR)assay was developed and subsequently evaluated for its effectiveness in simultaneously detecting multiple viral infections of Mink enteritis virus(MEV),Aleutian disease virus(ADV),Canine adenovirus(CAV).Based on the reports and sequences published in GenBank,three pairs of specific primers were designed according to the conservative compass.Three pairs of specific primers for each of these virus genomes MEV/ADV/CAV were amplified to detect 795 bp(MEV),451 bp(ADV),1 019 bp(CAV),res... |
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| Keywords: | multiplex PCR MEV ADV CAV |
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