| 一个甘蓝型油菜细胞质雄性不育相关基因克隆和表达分析 |
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| 引用本文： | 田琳琳,管荣展,张凤启,张红生. 一个甘蓝型油菜细胞质雄性不育相关基因克隆和表达分析[J]. 分子植物育种, 2008, 6(6) |
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| 作者姓名： | 田琳琳 管荣展 张凤启 张红生 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室,南京,210095;南京农业大学作物遗传与种质创新国家重点实验室,南京,210095;南京农业大学作物遗传与种质创新国家重点实验室,南京,210095;南京农业大学作物遗传与种质创新国家重点实验室,南京,210095 |
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| 基金项目： | 国家自然科学基金 ， 作物遗传与种质创新国家重点实验室资助项目 |
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| 摘 要： | 利用甘蓝型油菜与播娘蒿融合杂种中的不育株与甘蓝型油菜杂交、回交，得到细胞质雄性不育系NJ65A。根据pol和nap雄性不育高度相关的基因orf224和orf222设计兼并引物，从NJ65A植株中克隆得到一个长度为675bp的基因，命名为orf224-NJ65A。该基因编码224个氨基酸，氨基酸序列与orf224、orf222和orf220序列相似性分别为：70％、53％、60％。RT—PCR表达分析表明：orf224-NJ65A在所检测的组织中都有表达，但在花、花蕾和茎中的表达量较大。推测orf224-NJ65A与细胞质雄性不育有关。
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| 关 键 词： | 甘蓝型油菜 雄性不育 克隆 表达 |
Molecular Cloning and Characterization of a orf224-like Gene Related to Cytoplamic Male Sterility in Brassica napus L. |
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| Tian Linlin,Guan Rongzhan,Zhang Fengqi,Zhang Hongsheng. Molecular Cloning and Characterization of a orf224-like Gene Related to Cytoplamic Male Sterility in Brassica napus L.[J]. Molecular Plant Breeding, 2008, 6(6) |
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| Authors: | Tian Linlin Guan Rongzhan Zhang Fengqi Zhang Hongsheng |
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| Affiliation: | Tian Linlin Guan Rongzhan* Zhang Fengqi Zhang Hongsheng State Key Lab of Crops Genetics , Gemplasm Enhancement,Nanjing Agricultural University,Nanjing,210095 |
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| Abstract: | Backcrossing male sterile somatic hybrids between B. napus and Descurainia Sophia with B. napus, a cy- toplamic male sterile (CMS) line NJ65A was obtained. Using primers designed according to the CMS-related se- quences of orf224 and orf222 from pol and nap cytoplasms, respectively, a putative CMS-related gene with a com- plete open reading frame of 675 bp encoding a peptide of 224 amino acid residues, was isolated from NJ65A by PCR, and was named as orf224-NJ65A. Sequence alignment and phylogenetic analysi... |
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| Keywords: | Brassica napus L. CMS Cloning Expression |
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