不同成熟度茶籽外植体诱导发生体胚的研究

为了探究不同成熟度的茶籽作为外植体诱导体细胞胚胎发生的差异，本文选择成熟度依次递增的茶籽E1、E2、E3和E4作为外植体进行培养研究，结果表明：E4外植体接种成活率最高，但是诱导体细胞胚发生率较低。E1外植体在灭菌时耗损率相对较高，而且体胚诱导发生能力并非最佳。E2和E3外植体诱导分化体细胞胚的频率以及诱导获得的二级体胚的频率都较高，E最佳，E3次之。以上结果说明E2茶籽外植体可以作为诱导体胚发生的优良材料。

Somatic Embryo Development of Tea Seeds in Different Maturity

In order to explore the different rate of somatic embryogenesis induced from various maturity of tea seeds as explants,E1, E2, E3 and E4 named by its maturity from young to old was used as explants for culture, The results indicated that： E4 explants have the highest survival of seed inoculation, but have a low rate of induced somatic embryogenesis. E1 explants have the relatively high damage rate in disinfection and sterilization, and the capacity of somatic embryogenesis is not the best. The frequency of somatic embryogenesis from explants of E2 and E3, as well as its secondary somatic embryogenesis, is relatively high. The best is E2. All the above illuminated that explants of E2 should be a good choice for somatic embryogenesis of tea.