| 苜蓿体细胞胚发生的影响因素 |
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| 引用本文： | 高明 田子玉 谭旭华. 苜蓿体细胞胚发生的影响因素[J]. 牧草与饲料, 2006, 0(1): 12-14,31 |
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| 作者姓名： | 高明 田子玉 谭旭华 |
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| 作者单位： | [1]吉林省农科院经信中心,吉林公主岭136100 [2]吉林农业大学,长春130118 |
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| 摘 要： | 在组织培养过程中诱导苜蓿体细胞胚发生的关键因素有两个：一是植物材料，包括基因型和外植体类型；二是培养基成分。研究结果认为：紫花苜蓿体细胞胚发生能力较强，无菌苗子叶和下胚轴是发生率较高的外植体；2．0mg／L的2,4-D补加适宜浓度的NAA、KT和BA等激素是相对平衡的激素组合，也是必需的。添加一些有机物有利于提高体细胞胚发生率。
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| 关 键 词： | 苜蓿 体细胞胚发生 外植体 激素 |
| 收稿时间： | 2006-03-20 |
| 修稿时间： | 2006-03-20 |
Study on the Factors Affecting Alfalfa Embryos Occurrence |
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| GAO Ming, TIAN Zi-yu, TAN Xu-hua. Study on the Factors Affecting Alfalfa Embryos Occurrence[J]. , 2006, 0(1): 12-14,31 |
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| Authors: | GAO Ming TIAN Zi-yu TAN Xu-hua |
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| Affiliation: | 1. The Center of A gric ultural Economics and Information, Jilin Academy of A gric ultural Sciences, Gongzhuling 136100, China; 2.Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | There are two key factors inducing alfalfa embryos occurrence during tissue culture. One is plant material including genotype and explants type; another is culture medium component. The research result showed that alfalfa embryos occurrence ability is stronger, cotyledon with germfree seedling and the hypocotyl are external plant tissue type that has higher occurrence. It is necessary to append NAA, KT and BA with fitting concentration on 2,4-D with 2.0 mg/L.h is propitious to boost the embryos occurrence to append some organic matter. |
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| Keywords: | Alfalfa Embryos occurrence Explants Hormone |
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