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C-terminal heat shock protein 70 of Mycobacterium tuberculosis as a molecular adjuvant for DNA vaccination with the porcine circovirus type 2 ORF2 (capsid) gene in mice
Authors:Fang Fu  Huabin Tian  Xuesong Li  Yuekun Lang  Guangzhi Tong  Siguo Liu  Haizhong Li  Wei Wang  Xi Li  Xin Chen
Institution:1. Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences No. 427, Harbin 150001, China;2. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Shanghai 200241, China;1. Division of Veterinary Surgery, School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, University of Nottingham, Sutton Bonington Campus, Leicestershire LE12 5RD, UK;2. Royal Veterinary College, University of London, Hawkshead Campus, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL9 7TA, UK;1. Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China;2. Department of Anesthesiology, Xijing Hospital, The Fourth Military Medical University, Xi’an, China;3. Department of Anesthesiology and Intensive Care, Jintan Hospital, Jiangsu University, Changzhou, China;4. Department of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China
Abstract:Mycobacterium tuberculosis heat shock protein 70 (HSP70) and the peptide binding C-terminal portion of HSP70 (amino acids 359-610; HSP70c) exert an adjuvant effect when used in vaccines. To enhance the immunogenicity of a DNA vaccine against porcine circovirus type 2 (PCV2), recombinant plasmids encoding the PCV2 ORF2 (capsid) gene fused to full length hsp70 (pCA-TCH) or truncated C-terminal hsp70c (pCA-TCHc) were constructed. Immunisation of mice with pCA-TCHc induced higher serum immunoglobulin G antibody levels, stronger T helper 1 immune responses and lower PCV2 viral titres following challenge than immunisation with pCA-TCH or Cap plasmids only.
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