Expansion under hypoxic conditions enhances the chondrogenic potential of equine bone marrow-derived mesenchymal stem cells |
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| Authors: | Beatriz Ranera Ana Rosa Remacha Samuel Álvarez-Arguedas Tomás Castiella Francisco José Vázquez Antonio Romero Pilar Zaragoza Inmaculada Martín-Burriel Clementina Rodellar |
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| Institution: | 1. Department of Physics, SCE — Shamoon College of Engineering, Beer-Sheva 84100, Israel;2. Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel;3. Department of Virology and Developmental Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel |
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| Abstract: | Bone marrow-derived mesenchymal stem cells (BM-MSCs) are widely used in regenerative medicine in horses. Most of the molecular characterisations of BM-MSCs have been made at 20% O2, a higher oxygen level than the one surrounding the cells inside the bone marrow. The present work compares the lifespan and the tri-lineage potential of equine BM-MSCs expanded in normoxia (20% O2) and hypoxia (5% O2). No significant differences were found in long-term cultures for osteogenesis and adipogenesis between normoxic and hypoxic expanded BM-MSCs. An up-regulation of the chondrogenesis-related genes (COL2A1, ACAN, LUM, BGL, and COMP) and an increase of the extracellular sulphated glycosaminoglycan content were found in cells that were expanded under hypoxia. These results suggest that the expansion of BM-MSCs in hypoxic conditions enhances chondrogenesis in equine BM-MSCs. |
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