亚洲栽培稻程氏指数与SSR标记分类的比较分析

 选取具有代表性的100份亚洲栽培稻品种，应用程氏指数和109个SSR分子标记对它们进行分类。结果表明，等位酶Ⅰ群（典型籼稻）和Ⅵ A群（温带粳稻）程氏指数和SSR标记亚结构特征均十分明显，拟合度较高（73.3%～100%）。Mantel检测揭示，程氏指数与SSR分子标记进行栽培稻分类存在明显的正相关，两种方法各个材料间遗传距离的相关达极显著水平（r＝0.466，P<0.01）。遗传结构和聚类分析表明，在籼粳亚种水平上，程氏指数和SSR标记分类结果趋于一致，但在亚种内亚结构分析中，程氏指数偏差较大，SSR标记具有更高的准确性。 同时， SSR分析表明混合型材料多存在于Ⅱ群和Ⅵ群内部。

Comparison of Cheng's Index- and SSR Markers-based Classification of Asian Cultivated Rice

A total of one hundred cultivated rice accessions were grouped based on Cheng’s index and SSR polymorphism.The results showed that the subpopulation features of allozyme groupⅠ(indica) and allozyme group Ⅵ-A(temperate japonica) were obvious both classified by Cheng’s index and SSR marker,with goodness of fit ranging from 73.3% to 100%.Mantel test revealed that the Euclidean distance of Cheng’s index was significantly correlated with Nei’s genetic distance of 109 SSR loci(r=0.466,P<0.01).According to both model-based structure and UPGMA cluster analysis,the classification results at subspecies level based on Cheng’s index coincided with that on SSR marker.Within subspecies,the subpopulations classified by Cheng’s phenotypic characters showed lower goodness of fit than SSR marker.In addition,allozyme group Ⅱ(aus,boro) and allozyme group Ⅵ(temperate japonica,tropical japonica) had more mixture types based on SSR locus analysis.