琼脂及琼脂糖中3,6-内醚半乳糖的测定

本文研究了间苯二酚法对琼脂及琼脂糖样品中的3,6-内醚半乳糖的测定方法。实验中,根据间苯二酚试剂与3,6-内醚半乳糖和果糖的浓度在0.25μmol/mL以下时,具有近乎相同的标准比色曲线的原理,分别对测定波长、显色温度、显色时间和比色时间等检测条件进行优化。结果显示,用间苯二酚法测定3,6-内醚半乳糖的最佳检测条件为:测定波长554 nm、显色温度80°C、显色时间15 min;在该条件下,对琼脂及琼脂糖样品中的3,6-内醚半乳糖进行测定,方法的相对偏差为0.94%～1.27%,加标回收率为105.4%～111.04%。表明该方法操作简便,具有较高的准确性和重复性。

Determination of 3,6-anhydro galactose in agar and agarose

This paper studied the determination of resorcinol method on agar and agarose samples of 3,6-anhydro galactose.According to the principle that resorcinol reagent can color both with 3,6-anhydro galactose and fructose,when both concentrations are lower than 0.25 μmol/mL,they almost have the same standard colorimetric curve.This paper also optimized the determination wavelength,color temperature,color time and colorimetric time.The results showed that the optimum conditions were as follows： the wavelength was 554 nm,color temperature was 80°C and reaction time was 15 min.Under this condition,agar and agarose samples of 3,6-anhydro galactose were measured,and the relative deviation was 0.94% to 1.27%,the spiked recovery was 105.4% to 111.04%.It showed that the method was simple,and had high accuracy and repeatability.