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Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix |
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| Authors: | Talita F.B. Souza Silmara S. Sakamoto Gabriel T.N.M. Ferreira Roberto Gameiro Marcia Marinho Alexandre L. de Andrade Tereza C. Cardoso |
| Affiliation: | 1Laboratory of Animal Virology and Cell Culture, College of Veterinary Medicine, UNESP-Universidade Estadual Paulista, Aracatuba, SP 16050-680, Brazil.;2Small Animal Surgery Center, College of Veterinary Medicine, UNESP-Universidade Estadual Paulista, Aracatuba, SP 16050-680, Brazil. |
| Abstract: | Canine mesenchymal cells (MSCs) derived from Wharton''s jelly were co-cultured, then supplemented or not supplemented with platelet rich plasma (PRP) and demineralized bone matrix (DBM) to verify osteogenic differentiation. Osteoblastic differentiation followed by mineralized bone matrix production was found to be significantly higher (p < 0.05) when MSCs were associated with PRP/DBM in culture after 14-21-days of induction. Osteopontin and osteocalcin gene expression were significantly superior (p < 0.05) under the same culture conditions after 21 days of observation. In conclusion, addition of PRP to DBM co-cultured with MSCs successfully induced osteogenesis in vitro. |
| Keywords: | bone mesenchymal stem cells platelet-rich plasma umbilical cord |