Self-assembly of proteins into designed networks |
| |
Authors: | Ringler Philippe Schulz Georg E |
| |
Affiliation: | Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universit?t Freiburg, Albertstrasse 21, D-79104 Freiburg im Breisgau, Germany. |
| |
Abstract: | A C4-symmetric tetrameric aldolase was used to produce a quadratic network consisting of the enzyme as a rigid four-way connector and stiff streptavidin rods as spacers. Each aldolase subunit was furnished with a His6 tag for oriented binding to a planar surface and two tethered biotins for binding streptavidin in an oriented manner. The networks were improved by starting with composite units and also by binding to nickel-nitrilotriacetic acid-lipid monolayers. The mesh was adjustable in 5-nanometer increments. The production of a net with switchable mesh was initiated with the use of a calcium ion-containing beta-helix spacer that denatured on calcium ion depletion. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|