| 葱蝇ADH基因的克隆及序列分析(英文) |
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| 引用本文： | 陈春露,陈斌,司风玲,何正波. 葱蝇ADH基因的克隆及序列分析(英文)[J]. 农业科学与技术, 2012, 0(5): 1007-1010 |
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| 作者姓名： | 陈春露 陈斌 司风玲 何正波 |
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| 作者单位： | 重庆师范大学昆虫与分子生物学研究所重庆高校生物活性物质工程研究中心,重庆市动物生物学重点实验室 |
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| 基金项目： | Supported by National Natural Science Foundation of China (30870340,31071968);Scientific and Technological Research Project of Chongqing Municipal Education Commission (KJ100620);Key Project of Chongqing Normal University (2011XLZ12)~~ |
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| 摘 要： | [目的]对葱蝇(Delia antiqua)ADH基因进行克隆,并对其进行序列分析。[方法]通过RACE的方法克隆葱蝇ADH基因的cDNA序列,同时对该序列进行同源性分析、氨基酸序列比对和系统发育分析。[结果]试验获得的cDNA全长1088bp,其中ORF771bp,编码256个氨基酸,推测其相对分子质量为30.80kDa,等电点为8.22;通过该基因推导的氨基酸序列与其他物种的ADH进行相似性比较和系统发育分析,发现葱蝇与刺舌蝇(Glossina morsitans morsitans)氨基酸序列的同源性最高。[结论]该研究为ADH基因的进一步研究提供了基础。
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| 关 键 词： | 葱蝇 乙醇脱氢酶 ADH基因 序列分析 系统发育 |
cDNA Cloning and Sequence Analysis of ADH Gene in Delia antiqua |
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| Chunlu CHEN,Bin CHEN,Fengling SI,Zhengbo HE. cDNA Cloning and Sequence Analysis of ADH Gene in Delia antiqua[J]. Agricultural Science ＆ Technology, 2012, 0(5): 1007-1010 |
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| Authors: | Chunlu CHEN Bin CHEN Fengling SI Zhengbo HE |
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| Affiliation: | Institute of Entomology and Molecular Biology,Chongqing Engineering Research Center of Bioactive Substances,Chongqing Key Laboratory of Animal Biology,Chongqing Normal University,Chongqing 401331,China |
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| Abstract: | [Objective] This study aims to conduct cloning and sequence analysis of ADH gene in D.Antiqua.[Method] Full-length cDNA of ADH gene in D.antiqua was cloned by using RACE technology(GenBank access number:JQ666006).Analysis of the homology,characteristics and functional domains of ADH sequence and the phylogenetic relationship to other dipteran ADH were conducted.[Result] The full length of ADH cDNA is 1 088 bp containing a 771 bp of ORF,encoding 256 amino acids,with a calculated relative molecular weight of 30.80 kDa and a theoretical isoelectric point of 8.22.The deduced amino acid sequence shares the highest homology with Glossina morsitans morsitans based on homological analysis and phylogenetic analysis.[Conclusion] This study provides basis for further research of ADH gene. |
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| Keywords: | Delia antiqua ADH Sequence analysis Phylogenetic relationship |
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