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| 黄精核糖体灭活蛋白双元表达载体的构建与鉴定 | |
| 引用本文： | 常维山,Henry De Greve,翟静,Nele Buys. 黄精核糖体灭活蛋白双元表达载体的构建与鉴定[J]. 农业生物技术学报, 2004, 12(3): 351-352 |
| 作者姓名： | 常维山 Henry De Greve 翟静 Nele Buys |
| 作者单位： | 1. 山东农业大学动物科技学院,泰安,271018 2. 比利时自由大学免疫与分子生物学研究所,布鲁塞尔,1640 3. 泰山医学院基础医学部,泰安,271018 |
| 摘 要： | Ribosome-inactivating proteins (RIPs)have been known to have cytotoxic activity by cleaving a specific adenine residue of 28S rRNA. RIPs can be divided into, type 1 and type 2. Type 2 is a toxic protein that was consisted of two Gal/GalNAc-binding chains, A and B Chains that connected through a disulfide linkage. The A chain of RIP has RNA N-glycosidase activity to cleave a specific adenine base from ribosomal RNA, |
| 关 键 词： | 核糖体灭活蛋白 双元表达载体 基因 PCR DNA |
Construction of Binary Vector pGV4945 of Ribosome-Inactivating Protein Gene from Polygonatum multiflorum |
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| Abstract: | |
| Keywords: | ribosome-inactivating proteins binary vector construction |
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