| 毒芹诱导分化培养基筛选 |
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| 引用本文： | 王艳玲,李云霄. 毒芹诱导分化培养基筛选[J]. 湖北农业科学, 2010, 49(2) |
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| 作者姓名： | 王艳玲 李云霄 |
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| 作者单位： | 吉林农业科技学院,吉林,吉林,132100 |
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| 基金项目： | 吉林省教育厅科研项目 |
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| 摘 要： | 通过8种添加不同种类和不同浓度植物激素的培养基,对毒芹进行组织培养试验,研究了不同种类和不同浓度植物激素对毒芹诱导成活数的影响,筛选出较适合诱导毒芹分化的培养基。结果表明,MS+6-BA 2mg/L+NAA 0.3mg/L+2,4-D 0.5mg/L+GA3 0.7mg/L、MS+6-BA 2mg/L+NAA 0.3mg/L+2,4-D 0.7mg/L和MS+6-BA 2mg/L+NAA 0.5mg/L+2,4-D 0.5mg/L培养基对毒芹的诱导成活率较高。是适合毒芹诱导分化的培养基。
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| 关 键 词： | 毒芹 组织培养 |
Selection of Differential Medium for Cicuta virosa |
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| WANG Yan-ling,LI Yun-xiao. Selection of Differential Medium for Cicuta virosa[J]. Hubei Agricultural Sciences, 2010, 49(2) |
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| Authors: | WANG Yan-ling LI Yun-xiao |
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| Abstract: | The survival techniques of induction of Cicuta virosa was studied by using tissue culture methods and adopting MS basic medium,adding the different types and different concentrations of plant hormones,choosing non-polluting dedicate stem Cicuta virosa,then carrying out random trial of tissue culture.According to the trial results,E (MS+NAA0.3+2,4-D0.5mg/L+GA_3 0.7mg/L),F(MS+NAA0.3 mg/L+2,4-D0.7mg/L),G(MS+NAA0.5mg/L+2,4-D0.5mg/L)had higher survival rate of induction. |
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| Keywords: | NAA 2 4-D GA_3 Cicuta virosa tissue culture NAA 2.4-D GA_3 |
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