| 肉牛CAST基因分子多态性与肉品质性状相关性的研究 |
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| 引用本文： | 邓桂馨,张银花,梁鸿斌. 肉牛CAST基因分子多态性与肉品质性状相关性的研究[J]. 北京农学院学报, 2007, 22(3): 25-27 |
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| 作者姓名： | 邓桂馨 张银花 梁鸿斌 |
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| 作者单位： | 北京农学院,动物科学技术系,北京,102206;北京农学院,动物科学技术系,北京,102206;北京农学院,动物科学技术系,北京,102206 |
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| 基金项目： | 北京农学院三项基金资助项目 |
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| 摘 要： | 运用现代分子生物技术寻找影响牛肉嫩度和大理石纹性状的候选基因。选取7个牛品种（群体）共176头牛作为试验动物，利用PCR—RFLP方法研究钙激活蛋白酶抑制蛋白基因（calpastatin，CAST）第6内含子（intron6）内的遗传变异，并进行克隆测序，证实酶切位点突变。利用最小二乘线性模型进行基因性效应差异显著性检验，结果表明CAST基因对牛肉的嫩度有显著作用，这为CAST作为牛肉嫩度候选基因提供进一步根据。
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| 关 键 词： | 肉牛 CAST基因 PCR-RFLP 嫩度 大理石纹 相关分析 |
| 文章编号： | 1002-3186（2007）03-0025-03 |
| 修稿时间： | 2007-06-13 |
Relationship of Molecular Polymorphisms in Bovine CAST Gene with Beef Meat Quality Traits |
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| DENG Gui-xin,ZHANG Yin-hua,LIANG Hong-bin. Relationship of Molecular Polymorphisms in Bovine CAST Gene with Beef Meat Quality Traits[J]. Journal of Beijing Agricultural College, 2007, 22(3): 25-27 |
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| Authors: | DENG Gui-xin ZHANG Yin-hua LIANG Hong-bin |
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| Affiliation: | Department of Animal Science and Veterinary Medicine, Beijing University of Agriculture,Beijing 102206,China |
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| Abstract: | This study was aimed to detect the genentic variations of CAST gene intron6 by PCR-RFLP in a total of 176 cattle from 7 breeds.After the polymorphism fragments were cloned and sequenced,the single nucleotide position and genotypes were assigned as BB for which being with the restriction site in CAST.Analysis of variance was conducted and it showed that beef tenderness was significantly influenced by CAST gene. |
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| Keywords: | beef cattle CAST gene PCR-RFLP tenderness marbling correlative analysis |
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