利用组织培养技术获得烟草抗性材料的研究

烟草（Nicotiana tabacum L.)叶片在含有2mg/L 2,4-D 0.2mg/L KT的MS培养基上培养产生愈伤组织。用硫酸二乙酯（DES）进行诱变处理，其半致死浓度为0．34％。处理后的愈伤组织接种到含有15％聚乙二醇（PEG）的筛选培养基上，获得对水分胁迫具有抗性的愈伤组织，诱变率为5．4％。抗性愈伤组织与对照相比，相对电导率和丙二醛（MDA）含量差异均在极显著水平。

Study on Deriving Tobacco Resistant Material from Tissue Culture Technology

Callus of Tobacco(Nicotiana tabacum L.) was derived from leaves cultured on MS culture medium containing 2mg/L 2,4 D and 0.2 mg/L KT. Half death concentration of diethylsulfate (DES) which treatment for mutant inducement is 0.34%. When the treated callus inoculated on the selection medium contains 15% PEG, 5.4% of water stress resistant mutants were induced. Difference of the relative electrical conductivity and the content of malondialdehyde (MDA) between mutants and control reached very significant level.