内生环状芽孢杆菌Jcxy8对番茄灰霉病的防病机制研究

为明确从番茄植株体内筛选出的内生环状芽孢杆菌(Bacillus circulan)Jcxy8对番茄灰霉病菌的抑菌作用及防病的生理生化机制,采用平板打孔法测定了菌株Jcxy8对灰霉病菌(Botrytiscinerea)的拮抗力。结果表明:菌株Jcxy8对灰霉病菌的抑菌圈直径为35.6mm,抑菌圈边缘的产孢抑制率达到66.9%。当菌株培养滤液浓度为40%时,病菌孢子萌发完全被抑制。镜检发现抑菌圈周围的菌丝(或芽管)细胞消融,生长扭曲,中间或顶端膨大成泡囊状。Jcxy8菌株与灰霉病菌同时处理的番茄果体内可溶性蛋白含量比清水对照处理高12.7%,比单独接种灰霉病菌处理高39.1%;SOD、POD、CAT活性均较只经病菌处理低;O2-产生速率比清水对照和病菌处理低,而比菌株Jcxy8处理高。说明菌株Jcxy8对番茄果实有明显的诱导抗病作用。

Controlling mechanisms of tomato gray mould by endophytic bacterium Jcxy8 (Bacillus circulan)

To explore the inhibiting effect and physiological mechanism of endophytic bacteria (Bacillus circulans) Jcxy8 isolated from tomato against Botrytis cinerea pathogen,a series of analysis were carried out including inhibitory ability analysis via of punching-poles.Results show that Jcxy8 strain inhibits mycelial growth of B.cinerea at an average inhibitory circle diameter of 35.6 mm.Inhibition rate of spore production around inhibitory circles is 66.9%.B.cinerea spores do not germinate when Jcxy8 strain cult...