除草剂对不同种植年限柑橘园土壤氮转化过程及温室气体排放的影响

为探讨除草剂施用对柑橘园土壤氮转化及温室气体排放的影响,在实验室培养条件下,研究了0年(林地)、种植10年和30年的柑橘园土壤中分别添加除草剂草甘膦和丁草胺后,尿素态氮含量、硝化和反硝化作用以及温室气体排放的变化。研究结果表明,橘园土壤中尿素第1 d的水解率、氮肥硝化率、反硝化作用损失总量以及N_2O和CO_2排放量显著高于林地土壤(P0.05)。与10年橘园土壤相比,30年橘园土壤显著增加了尿素的水解速率、氮肥硝化率和CO_2排放量(P0.05),但二者的反硝化损失量没有显著差异。施用草甘膦和丁草胺都显著促进了林地土壤的尿素水解(P0.05),第1 d尿素态氮含量分别降低11.20%和12.43%;但对3种土壤氮肥的硝化率均没有明显影响。施用丁草胺显著降低了林地土壤的CO_2排放量(P0.05),对两种橘园土壤的CO_2排放没有明显影响,但明显增加了两种橘园土壤的N_2O排放总量(P0.05),分别比不施除草剂增加56.27%和85.41%;施用草甘膦对3种土壤的N_2O和CO_2排放均没有明显影响。可见,草甘膦和丁草胺的施用不会对柑橘园土壤的氮转化过程产生影响,但丁草胺显著增加了柑橘园土壤的N_2O排放。

Effects of herbicides on urea nitrogen transformation and greenhouse gas emission of soil in citrus orchards with different planting years

Soil microbes significantly influence the transformation and fate of nitrogen in soils by participating in the biology and biochemistry processes of soil nitrogen cycle. Research has shown that herbicides inhibit non-target soil microbes and the related biochemical processes. Therefore, herbicides have significant effects on plant uptake and utilization of nitrogen and its release from soil into the environment. This experiment aimed to explore the effects of herbicides on the transformation of urea nitrogen and greenhouse gas emissions in soils planted with citrus orchards for different years. It was important to evaluate the environmental safety of herbicides and nitrogen application in orchard soils. Thus a 2-factor, 3-level complete block design experimentwith herbicide factors of no herbicide, glyphosate of 10 mg·kg^-1 a.i. and butachlor of 10 mg·kg^-1 a.i.; and planting year factors of 0-year (woodland), 10-year and 30-year citrus orchard] was conducted under laboratory conditions with 200 mg(N)·kg^-1(dry soil) urea applied in each treatment. The results showed that urea hydrolysis rate, nitrification rate, denitrification loss and greenhouse gas emission of citrus orchard (10 and 30 years) were higher than those of the woodland (P < 0.05). Compared with woodland, the 10-year and 30-year citrus orchards increased soil total denitrification loss by 5.12 and 4.30 times, total N₂O emission by 7.80 and 2.74 times, and total CO₂ emission by 19.62% and 39.64%, respectively. Soil urea hydrolysis rate, nitrification rate and CO₂ emission in the 30-year citrus orchard were significantly greater than those in the 10-year citrus orchard. Compared with the 10-year citrus orchard soil, total CO₂ emission increased by 16.74%, but total denitrification loss was no significantly different in the 30-year citrus orchard soil. Glyphosate and butachlor significantly boosted urea hydrolysis in woodland soil, but had no effect on nitrification in the 30-year citrus orchard soil. Butachlor had significant negative effect on woodland CO₂ emission, but showed no effect on citrus orchard soil. Compared with the zero herbicide treatment, butachlor significantly (P < 0.05) boosted N₂O emission, respectively by 56.27% and 85.41% in the 10-year and 30-year citrus orchard soils. Glyphosate had no effect on soil total CO₂ and N₂O emission in soils under the three treatments of citrus planting year. Herbicides glyphosate and butachlor had no significant effect on nitrogen transformation in citrus orchard soils, but butachlor significantly boosted N₂O emission in citrus orchard soils.