| 桔梗胚珠培养初报 |
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| 引用本文： | 吴京姬,严一字,吴松权,朴雪梅,吴基日. 桔梗胚珠培养初报[J]. 北方园艺, 2010, 0(2): 154-157 |
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| 作者姓名： | 吴京姬 严一字 吴松权 朴雪梅 吴基日 |
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| 作者单位： | 延边农业科学院,生物技术研究所,吉林,龙井,133400;延边大学,农学院,吉林,龙井,133400 |
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| 基金项目： | 吉林省科技厅基础处基金资助项目(20040553) |
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| 摘 要： | 通过把从4种不同长度桔梗花蕾中取出的未受精胚珠接种在4种不同组合的培养基上诱导愈伤组织,并把诱导的愈伤组织转接到分化培养基上,试图得出单倍体植株。结果表明:诱导率最高(82.9%)的培养基组合是N6+2,4-D1mg/L+6-BA0.2mg/L;从长度为1.8cm花蕾中取出的胚珠诱导率最高(82.4%);在N6+6-BA1mg/L+NAA0.5mg/L分化培养基上最高分化率可达74.5%;经根尖压片检查染色体数目的结果,分化的绿苗中细弱的植株是单倍体植株。
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| 关 键 词： | 桔梗 胚珠培养 单倍体 |
Preliminary Study of Ovule Culture of Platycodon grandiflorum |
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| WU Jing-ji,YAN Yi-zi,WU Song-quan,PIAO Xu-emei,WU Ji-ri. Preliminary Study of Ovule Culture of Platycodon grandiflorum[J]. Northern Horticulture, 2010, 0(2): 154-157 |
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| Authors: | WU Jing-ji YAN Yi-zi WU Song-quan PIAO Xu-emei WU Ji-ri |
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| Affiliation: | 1.Yanbian Agricultural Research Institution of Biological;Longjing;Jilin 133400;2.Agricultural College of Yanbian University;Jilin 133400 |
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| Abstract: | Ovule of Platycodon grandiflorum growing to of different length was inoculated in four differentiation culture medium and induced callus,the induced callus was transfered in differentiation culture,trying to get haploidy in this study.The results showed that the culture medium of the highest(82.9%)induced rate of callus was N6+2,4-D 1 mg/L+6-BA 0.2 mg/L ;induced rate the highest(82.4%)from ovule to 1.8 cm length flower bud,the highest differentiation rate was 74.5%;in the culture of N6+6-BA 1 mg/L +NAA 0.5 ... |
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| Keywords: | platycodon grandiflorum ovule culture haploid |
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