Protective effect following intranasal exposure of goats to live Pasteurella multocida B:2 |
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Authors: | M Zamri-Saad Z A Ernie M Y Sabri |
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Institution: | (1) Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia |
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Abstract: | This study aimed to determine the effect of intranasal exposure to low doses of Pasteurella multocida B:2 on survival of goats challenged with high doses of the same organism. Eighteen goats were selected and divided into three
groups. Goats of group 1 were exposed intranasally twice, with a two-week interval, to 7× 106 cfu/ml of live P. multocida B:2. Goats of group 2 were not exposed to P. multocida B:2 but were kept together with the exposed group 1. Goats of group 3 remained as unexposed controls and were kept separated
from the other two groups. Serum samples were collected at weekly intervals to determine the antibody levels. At week 5 post
exposure, all goats were challenged subcutaneously with 3.7× 1010 cfu/ml of live P. multocida B:2. Following challenge exposure, 8 (67%) goats (4 goats from each of groups 1 and 2) were killed owing to haemorrhagic
septicaemia. Four goats were killed peracutely within 48 h post challenge, while the other four goats were killed acutely
between 2 and 4 days post challenge. None of the goats of group 3 were killed for haemorrhagic septicaemia. Goats of groups
1 and 2 showed significantly (p<0.05) higher antibody levels following the first intranasal exposure to P. multocida B:2. However, only group 1 retained the significantly (p<0.05) high antibody levels following a second intranasal exposure, and remained significantly (p<0.05) higher than groups 2 and 3 at the time of challenge. P. multocida B:2 was successfully isolated from various organs of goats that were killed between 1 and 4 days post challenge. |
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Keywords: | Intranasal Pasteurella multocida B:2 Goat |
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