抗多菌灵和乙霉威叶霉菌株可溶性蛋白和酯酶的电泳图谱研究

比较研究了对多菌灵、乙霉威表现不同抗性的番茄叶霉病菌菌株的可溶性蛋白和酯酶的电泳图谱。4种类型的菌株分别为: 对多菌灵、乙霉威均表现敏感(SS); 对多菌灵产生抗性, 而对乙霉威敏感(RS); 对多菌灵敏感, 而对乙霉威产生抗性(SR); 对二者均表现抗性(RR)。结果表明, 对多菌灵、乙霉威产生抗药性的田间突变菌株, 其可溶性蛋白质电泳图谱中谱带数均比敏感菌株明显减少; 抗乙霉威的菌株, 其酯酶电泳图谱类型均与其相应的敏感菌株的酯酶电泳图谱类似, 两者间差异主要表现为个别酶带强弱的变化, 酶带种类及酶带数差异极小; 对多菌灵抗性菌株酯酶电泳图谱中都存在一些抗性菌株特有的酶带, Rf0 .39、Rf0 .47、Rf0. 61, Rf0. 65、Rf0. 61五条酶带为抗多菌灵叶霉菌株所特有, 这些酯酶带的出现可能与抗性的产生直接相关。

Studies on the Electrophoretic Patterns of Soluble Proteins and Esterases of Fulria fulva Isolates with Different Resistance to Carbendazim and Diethofencarb

The electrophoretic patterns of soluble proteins and esterase isoenzymes of Fulria fulva (Cooke)Ciferri isolates with various response to carbendazim (Ben) and diethofencarb (NPC) were determined. The isolates tested were divided into 4 types, they were SS (sensitive to both chemicals), RS (resistant to Ben, sensitive to NPC), SR (sensitive to Ben, resistant to NPC) and RR (resistant to both chemicals), respectively. Results showed that the number of electrophoretic bands of soluble protein of RR isolate were significantly less than that of sensitive isolate. The electrophoretic patterns of esterase of NPC-resistant isolate were similar with that of NPC-sensitive isolate, only some esterase activity varied. Five particular bands (R f 0.39、R f 0.47、R f 0.61,R f 0.65、R f0.61) were found only in the electrophoretic patterns of esterase of Ben-resistant isolates, and these bands were considered to be correlative to the development of resistance.