溶藻弧菌外膜蛋白(Va-OMP)的免疫原性及免疫保护性

利用初步制备的溶藻弧菌外膜蛋白（outer membrane protein of Vibrio alginolyticus, Va-OMP）、溶藻弧菌蛋白分子量58 kD外膜蛋白（Va-OMP58）和溶藻弧菌（Vibrio alginolyticus, Va）灭活菌苗、溶藻弧菌脂多糖（lipopolysaccharides of Vibrio alginolyticus, Va-LPS）菌苗等进行主动保护性和被动保护性的实验比较，VaOMP、Va-OMP58免疫组小鼠免疫后用活菌攻击，免疫保护率为62.5%～86.7%,而Va-LPS组和Va灭活菌苗组的免疫保护率为50%～62.5%，对照组的免疫保护率仅为6.7%。用不同抗血清免疫小鼠，活菌攻击后，Va-OMP组存活率达到53.3%和66.7%，VaOMP58组次之，存活率为40%和50%，Va灭活菌组的存活率为33.3%和46.7%，对照组为13.3%。与对照组相比较，Va-OMP组差异较显著，保护效果较好。溶藻弧菌外膜蛋白和蛋白分子量58 kD外膜蛋白的保护性效果高于溶藻弧菌灭活菌苗和溶藻弧菌脂多糖的保护性效果，证明外膜蛋白（outer membrane protein, OMP）具有较强的免疫原性。迟发性超敏反应试验也证明，OMP能诱发变态反应，间接证明OMP具有较强的引起细胞介导免疫反应的能力。因此实验证明，Va-OMP、Va-OMP⁵⁸是能在小鼠产生体液免疫和细胞介导免疫的保护性抗原。

Immunogenicity and immunoprotection of outer membrane proteins of Vibrio alginolyticus

Outer membrane protein(OMP) is the main member of outer membrane,which is the unique structure of Gram-negative bacterium and plays great role in life function of bacterium.However,few researches were reported about immunogenicity and immunoprotection of outer membrane proteins of Vibrio alginolyticus(Va-OMP).In this paper,we prepared four vaccines about V.alginolyticus(Va) and outer membrane protein of V.alginolyticus.Comparing active and passive immunoprotection of outer membrane protein of V.alginolyticus,58 kD of outer membrane protein of V.alginolyticus(Va-OMP_(58)), inactive V.alginolyticus and lipopolysaccharides of V.alginolyticus(Va-LPS) vaccine,the survival percentage of group VaOMP and group Va-OMP_(58) was between 62.5%-86.7%.However,the survival percentage of group Va-LPS and group inactive Va was between 50%-62.5%.After being immunized with antiserum and challenged by Va,the survival percentage of group Va-OMP was 53.3% and 66.7%.It showed that Va-OMP vaccine and Va-OMP_(58) vaccine had higher immunogenicity than inactive vaccine and Va-LPS vaccine in mice.Moreover,outer membrane protein(OMP) can induce hypersensitivity reaction in mice by delayed-type hypersensitivity(DTH) test.All the results showed that Va-OMP may be the protective antigen which is capable of inducing both humoral immunity and cell-mediated immunity(CMI) in mice.The immunoprotection of OMP in Epinephelus fario is in process.The experiment provided the theory base for vaccine preparation of V.alginolyticus.It hopes that Va-OMP_(58) may be the protective antigen of V.alginolyticus and further be a candidate of the subunit vaccine.